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Geographic isolates of Lymantria dispar multiple nucleopolyhedrovirus: Genome sequence analysis and pathogenicity against European and Asian gypsy moth strainsAuthor(s): Harrison Robert L.; Daniel L. Rowley; Melody A. Keena
Source: Journal of Invertebrate Pathology. 137: 10-22.
Publication Series: Scientific Journal (JRNL)
Station: Northern Research Station
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DescriptionIsolates of the baculovirus species Lymantria dispar multiple nucleopolyhedrovirus have been formulated and applied to suppress outbreaks of the gypsy moth, L. dispar. To evaluate the genetic diversity in this species at the genomic level, the genomes of three isolates from Massachusetts, USA (LdMNPV-Aba624), Spain (LdMNPV-3054), and Japan (LdMNPV-3041) were sequenced and compared with four previously determined LdMNPV genome sequences. The LdMNPV genome sequences were collinear and contained the same homologous repeats (hrs) and clusters of baculovirus repeat orf (bro) gene family members in the same relative positions in their genomes, although sequence identities in these regions were low. Of 146 non-bro ORFs annotated in the genome of the representative isolate LdMNPV 5-6, 135 ORFs were found in every other LdMNPV genome, including the 37 core genes of Baculoviridae and other genes conserved in genus Alphabaculovirus. Phylogenetic inference with an alignment of the core gene nucleotide sequences grouped isolates 3041 (Japan) and 2161 (Korea) separately from a cluster containing isolates from Europe, North America, and Russia. To examine phenotypic diversity, bioassays were carried out with a selection of isolates against neonate larvae from three European gypsy moth (Lymantria dispar dispar) and three Asian gypsy moth (Lymantria dispar asiatica and Lymantria dispar japonica) colonies. LdMNPV isolates 2161 (Korea), 3029 (Russia), and 3041 (Japan) exhibited a greater degree of pathogenicity against all L. dispar strains than LdMNPV from a sample of Gypchek. This study provides additional information on the genetic diversity of LdMNPV isolates and their activity against the Asian gypsy moth, a potential invasive pest of North American trees and forests.
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CitationFajvan, M.A., and R.S. Morin. 2016. Monitoring the effects of an invasive insect (Adelges tsugae) on forest decline and landscape-level hydrologic processes. IN: Proceedings of the Geological Society of America Annual Meeting, Denver, CO September 24-28, 2016. Paper No.310-10. (Abstract) https://gsa.confex.com/gsa/2016AM/webprogram/Paper283319.html
KeywordsAsian gypsy moth, Baculovirus, LdMNPV, Lymantria dispar, gypsy moth, nucleopolyhedrovirus
- Classification, genetic variation and pathogenicity of Lymantria dispar nucleopolyhedrovirus isolates from Asia, Europe, and North America
- A comparison of the adaptations of strains of Lymantria dispar multiple nucleopolyhedrovirus to hosts from spatially isolated populations
- Potency of nucleopolyhedrovirus genotypes for European and Asian gypsy moth (Lepidoptera: Lymantriidae)
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