Skip to Main Content
Microbial lipid production from SPORL-pretreated Douglas fir by Mortierella isabellinaAuthor(s): S.M. Harde; Z. Wang; M. Horne; Junyong Zhu; X. Pan
Publication Series: Scientific Journal (JRNL)
Station: Forest Products Laboratory
Download Publication (876.0 KB)
DescriptionThe solid substrate and spent liquo robtained after SPORL pretreatment of Douglas fir residues were evaluated as substrates for the production of intracellular microbial lipid by Mortierella isabellina NRRL 1757. The production of lipid was investigated in a batch fermentation using different strategies viz. separate hydrolysis and fermentation,quasi-simultaneous saccharification and fermentation(Q-SSF),and whole slurry (solid substrate and spent liquor)saccharification and fermentation. The maximum lipid yield (0.21 g/gsugar) was obtained with Q-SSF of the solid substrate Nutrients upplementation was not necessary for lipid production from SPORL-pretreated Douglas fir. Detoxification of SPORL spent liquor with lime did remove the inhibitors and improved the fermentation for lipid production.The lipid produced had as imilar fatty acid compositional profile to those of vegetable oil,suggesting that it could have potential as asubstrate for biodiesel production.
- We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
- This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.
CitationHarde, S.M.; Wang, Z.; Horne, M.; Zhu, J.Y.; Pan, X. 2016. Microbial lipid production from SPORL-pretreated Douglas fir by Mortierella isabellina. Fuel. 175: 64-74.
KeywordsForest residue, Microbial lipid, SPORL pretreatment, Mortierella isabellina, Detoxification
- Batch fermentation options for high titer bioethanol production from a SPORL pretreated Douglas-Fir forest residue without detoxification
- SPORL pretreatment spent liquors enhance the enzymatic hydrolysis of cellulose and ethanol production from glucose
- Pilot-scale demonstration of SPORL for bioconversion of lodgepole pine to bioethanol and lignosulfonate
XML: View XML