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Mountain pine beetle infestation: GCxGCTOFMS and GC-MS of lodgepole pine (pinus contorta) acetone extractivesAuthor(s): Roderquita K. Moore; Michael Leitch; Erick Arellano-ruiz; Jonathon Smaglick; Doreen Mann
Source: In: Proceedings of the 18th ISWFPC (International Symposium on Wood, Fiber, and Pulping Chemistry). 4 p.
Publication Series: Paper (invited, offered, keynote)
Station: Forest Products Laboratory
PDF: Download Publication (172.0 KB)
DescriptionThe Rocky Mountains and western U.S. forests are impacted by the infestation of mountain pine beetles (MPB). MPB outbreak is killing pine and spruce trees at an alarming rate. These trees present a fuel build-up in the forest, which can result in catastrophic wildland fires. MPB carry blue-stain fungi from the genus Ophiostoma and transmit infection by burrowing into trees to lay eggs. Once attacked by a pine beetle, the host tree produces resinous compounds around the burrow in an effort to kill the invading insect. Mortality rate of the trees is less than one year. Lodgepole pine acetone extracts were separated and fractionated. These extractives were class of chemicals were characterized and identified using gas chromatography-mass spectrometry (GC-MS) and 2 dimensional GC (GCxGC). Solid phase extraction was used to fractionate the infected and uninfected extractive-samples into various chemical classes. The extractives from the sapwood (SW) and heartwood (HW) were separately fractionated. They were separated into polar (P) and nonpolar (NP) components. The NP component was further fractionated into acid (A) components, neutral (N) components and into individual chemical classes.
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CitationMoore, Roderquita K.; Leitch, Michael; Arellano-ruiz, Erick; Smaglick, Jonathon; Mann, Doreen. 2015. Mountain pine beetle infestation: GCxGCTOFMS and GC-MS of lodgepole pine (pinus contorta) acetone extractives. Proceedings of the 18th ISWFPC (International Symposium on Wood, Fiber, and Pulping Chemistry). 4 p.
KeywordsCharacterization of chemical classes, identification, fractionation, GC-MS, GCxGC, extractives, mountain pine beetle, infestation
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