Skip to Main Content
Use of NMR spectroscopy and magnetic resonance imaging for discriminating Juglans nigra L. seedsAuthor(s): John A. Vozzo; J.M. Halloin; T.G. Cooper; E.J. Potechen
Source: Seed Sci.& Technol., 24. 457-463
Publication Series: Miscellaneous Publication
PDF: View PDF (156 KB)
DescriptionBlack walnut (JuglamnigraL.) seeds are large and require stratification for germination. However, many seeds fail to germinate following stratification. Radiography can be used to select empty seeds, but cannot determine which full seeds will germinate. The objective of this study was to determine if any discrimination could bc achieved through use of nuclear magnetic resonance (NMR) spectroscopy or magnetic resonance imaging (MRI).of seeds. Both NMR spectroscopy and MRI were as effective as radiography for detecting empty seeds. NMR spectroscopy before stratification showed that most full seeds gave proton spectral peaks for both water and lipids; some full seeds, however, showed no major lipid peak and consistently failed to germinate following stratification. NMR spectra of seeds following stratification were similar to those obtained before stratification. Results of MRJ experiments mirrored those of spectroscopy experiments: seeds lacking large amounts of lipid produced images with very low intensity relative to those containing abundant lipid. Images of all embryos were more intense following stratification. Among seeds containing large amounts of lipid, germinable seeds were indistinguishable from non-germinable ones by either method.
- You may send email to firstname.lastname@example.org to request a hard copy of this publication.
- (Please specify exactly which publication you are requesting and your mailing address.)
- We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
- This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.
CitationVozzo, John A.; Halloin, J.M.; Cooper, T.G.; Potechen, E.J. 1996. Use of NMR spectroscopy and magnetic resonance imaging for discriminating Juglans nigra L. seeds. Seed Sci.& Technol., 24. 457-463
- In Vivo Digital Phyto Imaging (IDPI) in Juglans Nigra Seeds
- Effects of desiccation on temperate recalcitrant seeds: differential scanning calorimetry, gas chromatography, electron microscopy, and moisture studies on Quercus nigra and Quercus alba
- Biopolymer nanocomposite films reinforced with nanocellulose whiskers
XML: View XML