An environmental DNA assay for detecting Arctic grayling in the upper Missouri River basin, North AmericaAuthor(s): K. J. Carim; J. C. S. Dysthe; Michael Young; Kevin McKelvey; Michael Schwartz
Source: Conservation Genetics Resources. doi: 10.1007/s12686-016-0531-1.
Publication Series: Scientific Journal (JRNL)
Station: Rocky Mountain Research Station
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The upper Missouri River basin in the northwestern US contains disjunct Arctic grayling (Thymallus arcticus) populations of conservation concern. To assist efforts aimed at understanding Artic grayling distribution, we developed a quantitative PCR assay to detect the presence of Arctic grayling DNA in environmental samples. The assay amplified low concentrations of Arctic grayling DNA consistently, and did not amplify non-target species, including sympatric salmonid fishes.
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CitationCarim, K. J.; Dysthe, J. C. S.; Young, M. K.; McKelvey, K. S.; Schwartz, M. K. 2016. An environmental DNA assay for detecting Arctic grayling in the upper Missouri River basin, North America. Conservation Genetics Resources. doi: 10.1007/s12686-016-0531-1.
KeywordsThymallus arcticus, eDNA, invasive species, qPCR
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