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    Author(s): Philippe Remigi; Wendy Sutton; Paul Reeser; Everett Hansen
    Date: 2009
    Source: Gen. Tech. Rep. PSW-GTR-221. Albany, CA: U.S. Department of Agriculture, Forest Service, Pacific Southwest Research Station: 311-314
    Publication Series: General Technical Report (GTR)
    Station: Pacific Southwest Research Station
    PDF: Download Publication  (90.0 KB)

    Description

    Phytophthora species are best known as pathogens of agricultural crops, or invasive pathogens destroying forests. Little is known about indigenous species, especially in wild ecosystems. Previous work showed that Phytophthora species are relatively abundant in natural streams in forests, but the species present are poorly characterized, and their ecology is essentially unknown. The aim of this work was to compare methods for the collection, identification, and enumeration of Phytophthora spp. in streams. Three methods of isolate collection were compared (leaf baits, pear baits, and filtration), and species identification was carried out using morphological and growth characters, and by DNA sequencing two regions of the genome (ITS rDNA, mitochondrial COX spacer region). Stream sampling was conducted at five locations in the Oak Creek drainage near Corvallis Oregon.

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    Citation

    Remigi, Philippe; Sutton, Wendy; Reeser, Paul; Hansen, Everett. 2009. Characterizing the community of Phytophthora species in an Oregon forest stream. In: Goheen, E.M.; Frankel, S.J., tech. coords. Proceedings of the fourth meeting of the International Union of Forest Research Organizations (IUFRO) Working Party S07.02.09: Phytophthoras in forests and natural ecosystems. Gen. Tech. Rep. PSW-GTR-221. Albany, CA: U.S. Department of Agriculture, Forest Service, Pacific Southwest Research Station: 311-314.

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