A system for dosage-based functional genomics in poplarAuthor(s): Isabelle M. Henry; Matthew S. Zinkgraf; Andrew T. Groover; Luca Comai
Source: The Plant Cell. 27(9): 2370-2383
Publication Series: Scientific Journal (JRNL)
Station: Pacific Southwest Research Station
Download Publication (2.0 MB)
Altering gene dosage through variation in gene copy number is a powerful approach to addressing questions regarding gene regulation, quantitative trait loci, and heterosis, but one that is not easily applied to sexually transmitted species. Elite poplar (Populus spp) varieties are created through interspecific hybridization, followed by clonal propagation. Altered gene dosage relationships are believed to contribute to hybrid performance. Clonal propagation allows for replication and maintenance of meiotically unstable ploidy or structural variants and provides an alternative approach to investigating gene dosage effects not possible in sexually propagated species. Here, we built a genome-wide structural variation system for dosage-based functional genomics and breeding of poplar. We pollinated Populus deltoides with gamma-irradiated Populus nigra pollen to produce >500 F1 seedlings containing dosage lesions in the form of deletions and insertions of chromosomal segments (indel mutations). Using high-precision dosage analysis, we detected indel mutations in ∼55% of the progeny. These indels varied in length, position, and number per individual, cumulatively tiling >99% of the genome, with an average of 10 indels per gene. Combined with future phenotype and transcriptome data, this population will provide an excellent resource for creating and characterizing dosage-based variation in poplar, including the contribution of dosage to quantitative traits and heterosis.
- You may send email to email@example.com to request a hard copy of this publication.
- (Please specify exactly which publication you are requesting and your mailing address.)
- We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
- This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.
CitationHenry, Isabelle M.; Zinkgraf, Matthew S.; Groover, Andrew T.; Comai, Luca. 2015. A system for dosage-based functional genomics in poplar. The Plant Cell. 27(9): 2370-2383.
- Breeding poplars with durable resistance to Melampsora larici-populina leaf rust: a multidisciplinary approach to understand and delay pathogen adaptation
- Cottonwood: An American Wood
- Early rooting of dormant hardwood cuttings of Populus: analysis of quantitative genetics and genotype x environment interactions
XML: View XML