Skip to Main Content
U.S. Forest Service
Caring for the land and serving people

United States Department of Agriculture

Home > Search > Publication Information

  1. Share via EmailShare on FacebookShare on LinkedInShare on Twitter
    Dislike this pubLike this pub


    We attempted to develop new polymorphic SSR primer pairs in walnut using sequences derived from Juglans nigra L. genomic enriched library with GA repeat. The designed 94 SSR primer pairs were subjected to gradient PCR in 12 walnut cultivars to determine their optimum annealing temperatures and to determine whether they produce bands. Then, the primer pairs which had amplification in agarose gel were analyzed in capillary electrophoresis to determine their allele sizes. According to the gradient PCR and capillary electrophoresis results, 60.6% of the SSR primer pairs did not amplify any bands in agarose gel. Rest of the 37 primer pairs produced bands and their annealing temperatures and allele sizes were determined. From the amplified primer pairs, 18 of them were monomorphic, while 19 of them were polymorphic. As a result, 20.2% polymorphism was obtained from 94 SSR primer pairs tested in this study which had lower ratio when compared to the literature.

    Publication Notes

    • Check the Northern Research Station web site to request a printed copy of this publication.
    • Our on-line publications are scanned and captured using Adobe Acrobat.
    • During the capture process some typographical errors may occur.
    • Please contact Sharon Hobrla, if you notice any errors which make this publication unusable.
    • We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
    • This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.


    Topcu, Hayat; Coban, Nergiz; Woeste, Keith; Sutyemez, Mehmet; Kafkas, Salih. 2015. Developing new microsatellite markers in walnut (Juglans regia L.) from Juglans nigra genomic GA enriched library. Ekin Journal of Crop Breeding and Genetics. 1-2: 93-99.


    SSR, walnut, polymorphism, PCR

    Related Search

    XML: View XML
Show More
Show Fewer
Jump to Top of Page