Skip to Main Content
Biological screening of selected Pacific Northwest forest plants using the brine shrimp (Artemia salina) toxicity bioassayAuthor(s): Yvette M. Karchesy; Rick G. Kelsey; George Constantine; Joseph J. Karchesy
Source: SpringerPlus. 5(1): 135-
Publication Series: Scientific Journal (JRNL)
Station: Pacific Northwest Research Station
View PDF (812.0 KB)
DescriptionThe brine shrimp (Artemia salina) bioassay was used to screen 211 methanol extracts from 128 species of Pacific Northwest plants in search of general cytotoxic activity. Strong toxicity (LC50 < 100 μg/ml) was found for 17 extracts from 13 species, with highest activity observed for Angelica arguta roots at <10 μg/ml. Notably, four species of cedar trees and one of juniper in the family Cupressaceae dominated this group with LC50 for heartwood extracts ranging from 15 to 89 μg/ml. Moderate toxicity (LC50 100–500 μg/ml) was found in 38 extracts from 27 species, while weak toxicity (LC50 500–1000 μg/ml) was detected for 17 extracts in 16 species. There were 139 extracts from 99 species that were non-toxic (LC50 > 1000 μg/ml). Our subsequent studies of conifer heartwoods with strong activity confirm the assay’s value for identifying new investigational leads for materials with insecticidal and fungicidal activity.
- You may send email to firstname.lastname@example.org to request a hard copy of this publication.
- (Please specify exactly which publication you are requesting and your mailing address.)
- We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
- This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.
CitationKarchesy, Yvette M.; Kelsey, Rick G.; Constantine, George; Karchesy, Joseph J. 2016. Biological screening of selected Pacific Northwest forest plants using the brine shrimp (Artemia salina) toxicity bioassay. SpringerPlus. 5(1): 135-.
KeywordsBrine shrimp lethality, Artemia salina, Methanol extracts, Bioactivity
- Northwest forest plants defeat pests and diseases!
- Ex situ genetic conservation of vulnerable high elevation conifer species in the Pacific Northwest, USA
- Predicting post-fire tree mortality for 14 conifers in the Pacific Northwest, USA: Model evaluation, development, and thresholds
XML: View XML