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    The brine shrimp (Artemia salina) bioassay was used to screen 211 methanol extracts from 128 species of Pacific Northwest plants in search of general cytotoxic activity. Strong toxicity (LC50 < 100 μg/ml) was found for 17 extracts from 13 species, with highest activity observed for Angelica arguta roots at <10 μg/ml. Notably, four species of cedar trees and one of juniper in the family Cupressaceae dominated this group with LC50 for heartwood extracts ranging from 15 to 89 μg/ml. Moderate toxicity (LC50 100–500 μg/ml) was found in 38 extracts from 27 species, while weak toxicity (LC50 500–1000 μg/ml) was detected for 17 extracts in 16 species. There were 139 extracts from 99 species that were non-toxic (LC50 > 1000 μg/ml). Our subsequent studies of conifer heartwoods with strong activity confirm the assay’s value for identifying new investigational leads for materials with insecticidal and fungicidal activity.

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    Karchesy, Yvette M.; Kelsey, Rick G.; Constantine, George; Karchesy, Joseph J. 2016. Biological screening of selected Pacific Northwest forest plants using the brine shrimp (Artemia salina) toxicity bioassay. SpringerPlus. 5(1): 135-.


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    Brine shrimp lethality, Artemia salina, Methanol extracts, Bioactivity

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