Skip to Main Content
U.S. Forest Service
Caring for the land and serving people

United States Department of Agriculture

Home > Search > Publication Information

  1. Share via EmailShare on FacebookShare on LinkedInShare on Twitter
    Dislike this pubLike this pub

    Description

    We inoculated loblolly pines with bark beetle-associated fungi and a fungal cell wall component, chitosan, known to induce responses in some pines and many other plants. Trees in Florida were inoculated with Leptographium procerum, L. terebrantis, Ophiostoma minus, or chitosan. Trees in Louisiana were inoculated with O. minus, Entomocorticium sp. A, or Ceratocystiopsis ranaculosus. In both Florida and Louisiana, mechanical wounds served as controls. Treatment responses were sampled after 3 weeks, and all produced uniform responses across trees. Inoculations with E. sp. A and C. ranaculosus appeared similar to controls. Inoculations with L. procerum produced slightly higher levels of host damage. Loblolly pine responded similarly to chitosan and pathogenic bark beetle-associated fungi (O. minus and L. terebrantis), producing high levels of phenolic compounds and cell hydrolysis in the callus. In addition, callus inoculated with O. minus appeared extremely disrupted and "stringy." Chitosan inoculations resulted in no hydrolysis, but produced extremely high levels of phenolics deposition, as well as noticeable periderm formation. Our results reveal possible morphological mechanisms for pine secondary response to these fungi and suggest that chitosan may have potential as a stable material for testing variability in this response.

    Publication Notes

    • You may send email to pubrequest@fs.fed.us to request a hard copy of this publication.
    • (Please specify exactly which publication you are requesting and your mailing address.)
    • We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
    • This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.

    Citation

    Klepzig, Kier D.; Walkinshaw, Charles H. 2003. Cellular response of loblolly pine to wound inoculation with bark beetle-associated fungi and chitosan. Res. Pap. SRS-30.Asheville, NC: U.S. Department of Agriculture, Forest Service, Southern Research Station. 9p.

    Keywords

    Leptographium, Ophiostoma, resin, resistance, southern pine beetle

    Related Search


    XML: View XML
Show More
Show Fewer
Jump to Top of Page
https://www.fs.usda.gov/treesearch/pubs/5322