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Cross amplification of 15 SSR markers in the genus FraxinusAuthor(s): Andrea G. Noakes; Teodora Best; Margaret E. Stanton; Jennifer Koch; Jeanne Romero-Severson
Source: Conservation Genetics Resources. 6(4): 969-970.
Publication Series: Scientific Journal (JRNL)
Station: Northern Research Station
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DescriptionAsh (Fraxinus, Oleaceae) species occur on most continents, within a wide range of forest tree communities. Emerald ash borer, Agrilus planipennis Fairmaire (Coleoptera: Buprestidae), introduced into the U.S. from Asia in the late twentieth century, has caused widespread mortality, primarily in green ash, Fraxinus pennsylvanica Marsh. (Section: Melioides) and now impacts other North American ash species. The development and successful reintroduction of resistant trees requires genetic tools to evaluate population dynamics and aid in species identification. Here, we report 15 novel EST-SSR markers developed in green ash, most of which amplify and are polymorphic across 18 species of Fraxinus, including six species native to North America. The high average polymorphism information content (0.741) and allelic richness (15.3) revealed in six disparate populations of green ash indicate that these markers also have utility for investigating population dynamics in this species.
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CitationNoakes, Andrea G.; Best, Teodora; Stanton, Margaret E.; Koch, Jennifer; Romero-Severson, Jeanne. 2014. Cross amplification of 15 SSR markers in the genus Fraxinus. Conservation Genetics Resources. 6(4): 969-970.
KeywordsFraxinus pennsylvanica, EST-SSRs, heterologous markers, green ash
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