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The copper-transporting ATPase pump and its potential role in copper-toleranceAuthor(s): Katie Ohno; C.A. Clausen; Frederick Green; G. Stanosz
Source: The International Research Group on Wood Protection, section 1, Biology, IRG/WP 16-10859.
Publication Series: Paper (invited, offered, keynote)
Station: Forest Products Laboratory
PDF: Download Publication (178.0 KB)
DescriptionCopper-tolerant brown-rot decay fungi exploit intricate mechanisms to neutralize the efficacy of copper-containing preservative formulations. The production and accumulation oxalate is the most widely recognized theory regarding the mechanism of copper-tolerance in these fungi. The role of oxalate, however, may be only one part of a series of necessary components required for this complex mechanism. Annotation of the Fibroporia radiculosa genes involved in copper-tolerance characterized a subset of proteins, three copper-transporting ATPase pumps, which regulate copper concentrations inside the fungal cell by exporting excess copper ions. The goal of this study was to determine the relevance of copper-transporting ATPase pumps in the mechanism of F. radiculosa copper-tolerance. Southern pine test blocks were pressure-treated with 0.6%, 1.2% and 2.4% ammoniacal copper citrate and subjected to a copper-tolerant strain of F. radiculosa and a copper-sensitive strain of Gloeophyllum trabeum in decay tests over a four week period. Untreated Southern pine test blocks subjected to both test fungi served as controls. Expression levels of three copper-transporting ATPase pumps were evaluated each week by qRT-PCR. F. radiculosa showed up regulation of all three ATPase pumps when exposed to the copper treatments over the course of this study. G trabeum showed down-regulation of ATPase1 and ATPase2 and no expressions of ATPase3 when exposed to the copper treatments over the course of this study. Up-regulation of the three ATPase pumps can be correlated to the ability of F. radiculosa to decay copper-treated wood (1% weight loss at week 4). Preliminary results indicate these three ATPase pumps function as an essential component of the complex mechanism of copper-tolerance utilized by F. radiculosa.
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CitationOhno, Katie M.; Clausen, C.A.; Green III, F.; Stanosz, G. 2016. The copper-transporting ATPase pump and its potential role in copper-tolerance. In. Proceedings IRG Annual Meeting (ISSN 2000-8953). The International Research Group on Wood Protection; IRG/WP 16-10859, Section 1, Biology. pp, 1-15.
Keywordsbrown-rot decay, copper tolerance, copper-transporting ATPase pump, Fibroporia radiculosa, gene expression
- Gene Expression Analysis of Three Putative Copper-Transporting ATPases in Copper-Tolerant Fibroporia radiculosa
- Untreated and copper-treated wood soaked in sodium oxalate: effects of decay by copper-tolerant and copper-sensitive fungi
- Insights into the mechanism of copper-tolerance in Fibroporia radiculosa: The biosynthesis of oxalate
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