Skip to Main Content
Synchrotron-based X-ray fluorescence microscopy enables multiscale spatial visualization of ions involved in fungal lignocellulose deconstructionAuthor(s): Grant T. Kirker; Samuel Zelinka; Sophie-Charlotte Gleber; David Vine; Lydia Finney; Si Chen; Young Pyo Hong; Omar Uyarte; Stefan Vogt; Jody Jellison; Barry Goodell; Joseph E. Jakes
Source: Scientific Reports, Vol. 7:1-15.
Publication Series: Scientific Journal (JRNL)
Station: Forest Products Laboratory
Download Publication (3.0 MB)
DescriptionThe role of ions in the fungal decay process of lignocellulose biomaterials, and more broadly fungal metabolism, has implications for diverse research disciplines ranging from plant pathology and forest ecology, to carbon sequestration. Despite the importance of ions in fungal decay mechanisms, the spatial distribution and quantification of ions in lignocellulosic cell walls and fungal hyphae during decay is not known. Here we employ synchrotron-based X-ray fluorescence microscopy (XFM) to map and quantify physiologically relevant ions, such as K, Ca, Mn, Fe, and Zn, in wood being decayed by the model brown rot fungus Serpula lacrymans. Two-dimensional XFM maps were obtained to study the ion spatial distributions from mm to submicron length scales in wood, fungal hyphae with the dried extracellular matrix (ECM) from the fungus, and Ca oxalate crystals. Three-dimensional ion volume reconstructions were also acquired of wood cell walls and hyphae with ECM. Results show that the fungus actively transports some ions, such as Fe, into the wood and controls the distribution of ions at both the bulk wood and cell wall length scales. These measurements provide new insights into the movement of ions during decay and illustrate how synchrotron-based XFM is uniquely suited study these ions.
- We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
- This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.
CitationKirker, Grant; Zelinka, Sam; Gleber, Sophie-Charlotte; Vine, David; Finney, Lydia; Chen, Si; Hong, Young Pyo; Uyarte, Omar; Vogt, Stefan; Jellison, Jody; Goodell, Barry; Jakes, Joseph E. 2017. Synchrotron-based X-ray fluorescence microscopy enables multiscale spatial visualization of ions involved in fungal lignocellulose deconstruction. Scientific Reportsl. 7:41798. pp. 1-15.
KeywordsX-ray fluorescence microscopy, Brown rot fungi, Wood decay, Tomography
- Fungal–copper interactions in wood examined with large field of view synchrotron-based X-ray fluorescence microscopy
- Distribution and oxidation state of copper in the cell walls of treated wood examined by synchrotron based XANES and XFM
- Nano-scale investigation of the association of microbial nitrogen residues with iron (hydr)oxides in a forest soil O-horizon
XML: View XML