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    Author(s): Christa Conforti
    Date: 2017
    Source: Proceedings of the sudden oak death sixth science symposium. Gen. Tech. Rep. GTR-PSW-255. Albany, CA: U.S. Department of Agriculture, Forest Service, Pacific Southwest Research Station: 65.
    Publication Series: General Technical Report (GTR)
    Station: Pacific Southwest Research Station
    PDF: Download Publication  (162.0 KB)

    Description

    A Phytophthora cactorum-infected nursery crop of Ceanothus thyrsiflorus was used to evaluate three Phytophthora monitoring methods. The Phytophthora detection level of three non-destructive sampling methods was quantified and compared to the detection level of destructive sampling. Non-destructive methods were (a) composite soil/root samples, pear-baited, (b) effluent samples, pear-baited, and (c) Agdia ImmunoStrip tests run on root samples. Time and expense of each method were also tracked. The baited soil/root sample method was the least labor intensive and most cost-effective method to test a large number of plants at our nursery. It also allows for species-level identification of Phytophthora, and has no risk of false positives. But detection level was 60%, so we have decided to combine it with destructive sampling, for an integrated monitoring approach.

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    Citation

    Conforti, Christa. 2017. How do we know if plants in our nursery have Phytophthora? Detection methods and an integrated approach to monitoring. In: Frankel, Susan J.; Harrell, Katharine M., tech. coords. Proceedings of the sudden oak death sixth science symposium. Gen. Tech. Rep. GTR-PSW-255. Albany, CA: U.S. Department of Agriculture, Forest Service, Pacific Southwest Research Station: 65.

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