Skip to Main Content
U.S. Forest Service
Caring for the land and serving people

United States Department of Agriculture

Home > Search > Publication Information

  1. Share via EmailShare on FacebookShare on LinkedInShare on Twitter
    Dislike this pubLike this pub
    Author(s): Corina Junker; Sabine Werres
    Date: 2017
    Source: Proceedings of the sudden oak death sixth science symposium. Gen. Tech. Rep. GTR-PSW-255. Albany, CA: U.S. Department of Agriculture, Forest Service, Pacific Southwest Research Station: 75.
    Publication Series: General Technical Report (GTR)
    Station: Pacific Southwest Research Station
    PDF: Download Publication  (176.0 KB)

    Description

    Bait tests are very helpful for diagnosis of Phytophthora in for example soil, substrate, water, sediment, and rootball samples (Werres and others 2014). By attracting the motile zoospores of the Phytophthora species with the baits these pathogens can be separated from other organisms. Bait tests are simple and cost effective and - compared with other diagnostic techniques - they are much more independent on the varying sample quality. Furthermore, bait tests can be used for evaluating big sample quantities.

    Within the preparation of laboratory accreditation according to ISO standard ISO/IEC 17025 the European Plant Protection Organization (EPPO) recommends the validation of laboratory methods (“Specific requirements for laboratories preparing accreditation for plant pest diagnostic activity” EPPO standard PM 7/98; PM 7/76). In this guideline, detailed instructions on test verification are given, like analytical sensitivity and specificity, selectively, repeatability, and reproducibility.

    Within the European project “Responses of European Forests and Society to Invasive Pathogens” (RESIPATH, http://www.slu.se/resipath) the bait test with rhododendron leaves will be validated according to the EPPO guidelines. For the validation the standard protocol with detached rhododendron leaves according to Themann and and Werres (1998, 2000) will be used. First results of the different validation steps will be presented.

    Publication Notes

    • You may send email to psw_communications@fs.fed.us to request a hard copy of this publication.
    • (Please specify exactly which publication you are requesting and your mailing address.)
    • We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
    • This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.

    Citation

    Junker, Corina; Werres, Sabine. 2017. Validation of the bait test with Rhododendron leaves for Phytophthora diagnosis. In: Frankel, Susan J.; Harrell, Katharine M., tech. coords. Proceedings of the sudden oak death sixth science symposium. Gen. Tech. Rep. GTR-PSW-255. Albany, CA: U.S. Department of Agriculture, Forest Service, Pacific Southwest Research Station: 75.

    Related Search


    XML: View XML
Show More
Show Fewer
Jump to Top of Page
https://www.fs.usda.gov/treesearch/pubs/54073