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    Author(s): Gregory J. Fischer; William Bacon; Jun Yang; Jonathan M. Palmer; Taylor Dagenais; Bruce D. Hammock; Nancy P. Keller
    Date: 2017
    Source: Frontiers in Microbiology
    Publication Series: Scientific Journal (JRNL)
    Station: Northern Research Station
    PDF: Download Publication  (2.0 MB)

    Description

    The opportunistic human pathogen Aspergillus fumigatus initiates invasive growth through a programmed germination process that progresses from dormant spore to swollen spore (SS) to germling (GL) and ultimately invasive hyphal growth. We find a lipoxygenase with considerable homology to human Alox5 and Alox15, LoxB, that impacts the transitions of programmed spore germination. Overexpression of loxB (OE::loxB) increases germination with rapid advance to the GL stage. However, deletion of loxBloxB) or its signal peptide only delays progression to the SS stage in the presence of arachidonic acid (AA); no delay is observed in minimal media. This delay is remediated by the addition of the oxygenated AA oxylipin 5-hydroxyeicosatetraenoic acid (5-HETE) that is a product of human Alox5. We propose that A. fumigatus acquisition of LoxB (found in few fungi) enhances germination rates in polyunsaturated fatty acid-rich environments.

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    Citation

    Fischer, Gregory J.; Bacon, William; Yang, Jun; Palmer, Jonathan M.; Dagenais, Taylor; Hammock, Bruce D.; Keller, Nancy P. 2017. Lipoxygenase activity accelerates programmed spore germination in Aspergillus fumigatus. Frontiers in Microbiology. 8: 831. 14 p. http://dx.doi.org/10.3389/fmicb.2017.00831.

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    Keywords

    Aspergillus fumigatus, spore germination, arachidonic acid, lipoxygenase activity, linoleic acid

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