Skip to Main Content
U.S. Forest Service
Caring for the land and serving people

United States Department of Agriculture

Home > Search > Publication Information

  1. Share via EmailShare on FacebookShare on LinkedInShare on Twitter
    Dislike this pubLike this pub
    Author(s): Ningxia Du; Xin Liu; Yun Li; Shouyi Chen; Jinsong Zhang; Da Ha; Wenguang Deng; Chunkui Sun; Yingzhi Zhang; Paula M Pijut
    Date: 2012
    Source: Plant Cell Tissue and Organ Culture. 108: 181-189.
    Publication Series: Scientific Journal (JRNL)
    Station: Northern Research Station
    PDF: Download Publication  (352.0 KB)


    Soil salinity can be a limiting factor for productivity in agriculture and forestry. In order to fully utilize saline lands productively in plantation forestry for pulp production, the genetic modification of tree species for salt tolerance may be required. The AhDREB1 gene, a DREB-like transcription factor gene, was transferred into Populus tomentosa by Agrobacterium-mediated transformation. Transgenic plants were regenerated and selected using a two-step process; first on Murashige and Skoog (MS) basal medium containing 4.44 µM 6-benzyladenine (BA), 1.61 µM a-naphthaleneacetic acid (NAA), 30 mg 1-1 kanamycin, and 250 mg 1-1 ceftomine, and then enhanced selection on medium with 50 mg 1-1 kanamycin. The putative transformants were confirmed by polymerase chain reaction (PCR) and Southern hybridization for the AhDREB1 gene. Transgenic plants were rooted on half-strength MS medium containing 5.71 µM indole-3-acetic acid (IAA), 1.61 µM NAA, 2 g 1-1 sucrose, and 5 g 1-1 agar. The salt tolerance of transgenic plants in pots in the greenhouse showed a survival rate of 100, 100, 84.4, and 44.4% after watering with a solution of 34.2, 68.4, 102.7, and 136.9 mM NaCl, respectively.

    Publication Notes

    • Check the Northern Research Station web site to request a printed copy of this publication.
    • Our on-line publications are scanned and captured using Adobe Acrobat.
    • During the capture process some typographical errors may occur.
    • Please contact Sharon Hobrla, if you notice any errors which make this publication unusable.
    • We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
    • This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.


    Du, Ningxia; Liu, Xin; Li, Yun; Chen, Shouyi; Zhang, Jinsong; Ha, Da; Deng, Wenguang; Sun, Chunkui; Zhang, Yingzhi; Pijut, Paula M. 2012. Genetic transformation of Populus tomentosa to improve salt tolerance. Plant Cell Tissue and Organ Culture. 108: 181-189.


    Google Scholar


    AhDREB1, Chinese white poplar, Genetic engineering, Transgenic

    Related Search

    XML: View XML
Show More
Show Fewer
Jump to Top of Page