Skip to Main Content
Potential concerns with analytical methods used for the detection of Batrachochytrium salamandrivorans from archived DNA of amphibian swab samples, Oregon, USAAuthor(s): Deborah D. Iwanowicz; William B. Schill; Deanna H. Olson; Michael J. Adams; Christine Densmore; R. Scott Cornman; Cynthia Adams; Jr. Figiel; Chauncey W. Anderson; Andrew R. Blaustein; Tara Chestnut
Source: Herpetological Review. 48(2): 352–355.
Publication Series: Scientific Journal (JRNL)
Station: Pacific Northwest Research Station
PDF: Download Publication (188.0 KB)
DescriptionWe report on the results of surveillance for the salamander chytrid fungus, Batrachochytrium salamandrivorans (Bsal). Two samples from archived DNA used in a previous chytrid study in Oregon were Bsal-positive, alerting us to the first potential finding of this pathogen in North America, infecting American bullfrogs and rough-skinned newts. To confirm the finding, several additional diagnostic tests were conducted, with results that did not definitively confirm our positive finding and our gene sequencing suggested our DNA had differences from the European Bsal fungus. Insufficient sample quantity precluded further analyses. Because our results are also consistent with sample contamination, we cannot rule out a false positive result. We provide recommendations for diagnostic analyse for future Bsal testing, and follow-up surveillance to better understand if Bsal occurs at our study populations, or to assay if a similar yet unidentified fBsal-like pathogen might have been detected.
- Visit PNW's Publication Request Page to request a hard copy of this publication.
- We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
- This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.
CitationIwanowicz, Deborah D.; Schill, William B.; Olson, Deanna H.; Adams, Michael J.; Densmore, Christine; Cornman, R. Scott; Adams, Cynthia; Figiel, Jr., Chester; Anderson, Chauncey W.; Blaustein, Andrew R.; Chestnut, Tara. 2017. Potential concerns with analytical methods used for the detection of Batrachochytrium salamandrivorans from archived DNA of amphibian swab samples, Oregon, USA. Herpetological Review. 48(2): 352–355.
Keywordsdisease, salamanders, frogs, chytrid fungus
- Effects of the Chytrid fungus on the Tarahumara frog (Rana tarahumarae) in Arizona and Sonora, Mexico
- DNA-based detection of the fungal pathogen Geomyces destructans in soils from bat hibernacula
- A field-portable diagnostic approach confirms Laurel Wilt Disease diagnosis in minutes instead of days
XML: View XML