Skip to Main Content
Comparison of RAPD Linkage Maps Constructed For a Single Longleaf Pine From Both Haploid and Diploid Mapping PopulationsAuthor(s): Thomas L. Kubisiak; C.Dana Nelson; W.L. Name; M. Stine
Source: Forest Genetics 3(4):203-211, 1996
Publication Series: Miscellaneous Publication
PDF: View PDF (448 KB)
DescriptionConsiderable concern has been voiced regarding the reproducibility/transferability of RAPD markers across different genetic backgrounds in genetic mapping experiments. Therefore, separate gametic subsets (mapping populations) were used to construct individual random amplified polymorphic DNA (RAPD) linkage maps for a single longleaf pine (Pinus palustris Mill.). A haploid mapping population consisting of megagametophytic DNAs Tom 88 wind-pollinated seeds of longleafpine (clone 3-356), and a diploid population including 86 F1 progeny fom a controlled cross [longleaf pine 3-356 (female) × slash pine H-28 (male)] were employed. Seventy-one, RAPD primers selected for this study identified a total of 137 mapped loci in longleaf pine 3-356 based on amplification of megagametophytic DNAs. Of the 137 loci useful for comparative purposes, 62 loci (45.30%) could not be scored when DNAs of F1 progeny from the controlled cross were amplified. Of the 75 loci that were storable, 26 loci (34.7%) were fixed in slash pine H-28, and 49 (65.3%) were segregating (42 1:1, and seven 3:1). Comparisons were made using the 49 loci common to both the haploid-and diploid-based maps. The 49 loci allowed us to determine homologous counterparts maps. Orders were conserved for those groupscontaining three or more loci. Genetic distance estimates were found vary considerably, but not in anysystematic manner.
- You may send email to email@example.com to request a hard copy of this publication.
- (Please specify exactly which publication you are requesting and your mailing address.)
- We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
- This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.
CitationKubisiak, Thomas L.; Nelson, C.Dana; Name, W.L.; Stine, M. 1996. Comparison of RAPD Linkage Maps Constructed For a Single Longleaf Pine From Both Haploid and Diploid Mapping Populations. Forest Genetics 3(4):203-211, 1996
Keywordsrandom amplified polymorphic DNAs (RAPDs), Pinus palustris Mill., genetic linkage maps, megagametophytic DNA
- RAPD linkage mapping in a longleaf pine × slash pine F1 family
- Mapping quantitative trait loci controlling early growth in a (longleaf pine × slash pine) × slash pine BC1 family
- Scar markers in a longleaf pine x slash pine F1 family
XML: View XML