Skip to Main Content
The activity of phenoloxidase in haemolymph plasma is not a predictor of Lymantria dispar resistance to its baculovirusAuthor(s): Nikita S. Kasianov; Irina A. Belousova; Sergey V. Pavlushin; Ivan M. Dubovskiy; John D. Podgwaite; Vyacheslav V. Martemyanov; Stanislav A. Bakhvalov; Erjun Ling
Source: PLOS ONE. 12(8): e0183940. 16 p.
Publication Series: Scientific Journal (JRNL)
Station: Northern Research Station
Download Publication (548.0 KB)
DescriptionHost innate immunity is one of the factors that determines the resistance of insects to their entomopathogens. In the research reported here we studied whether or not phenoloxidase (PO), a key enzyme in the melanogenesis component of humoral immunity of insects, plays a role in the protection of Lymantria dispar larvae from infection by L. dispar multiple nucleopolyhedrovirus. We studied two types of viral infection: overt and covert. The following lines of investigation were tested: i) the intravital individual estimation of baseline PO activity in haemolymph plasma followed by virus challenging; ii) the specific inhibition of PO activity in vivo by peroral treatment of infected larvae with phenylthiourea (PTU), a competitive inhibitor of PO; iii) the evaluation of PO activity in the haemolymph plasma after larval starvation. Starvation is a stress that activates the covert infection to an overt form. All of these experiments did not show a relationship between PO activity in haemolymph plasma of L. dispar larvae and larval susceptibility to baculovirus. Moreover, starvation-induced activation of covert viral infection to an overt form occurred in 70 percent of virus-carrying larvae against the background of a dramatic increase of PO activity in haemolymph plasma in the insects studied. Our conclusion is that in L. dispar larvae PO activity is not a predictor of host resistance to baculovirus.
- Check the Northern Research Station web site to request a printed copy of this publication.
- Our on-line publications are scanned and captured using Adobe Acrobat.
- During the capture process some typographical errors may occur.
- Please contact Sharon Hobrla, firstname.lastname@example.org if you notice any errors which make this publication unusable.
- We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
- This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.
CitationKasianov, Nikita S.; Belousova, Irina A.; Pavlushin, Sergey V.; Dubovskiy, Ivan M.; Podgwaite, John D.; Martemyanov, Vyacheslav V.; Bakhvalov, Stanislav A.; Ling, Erjun. 2017. The activity of phenoloxidase in haemolymph plasma is not a predictor of Lymantria dispar resistance to its baculovirus. PLOS ONE. 12(8): e0183940. 16 p.
- Contributions of immune responses to developmental resistance in Lymantria dispar challenged with baculovirus
- Interaction between a Nosema sp. (Microspora: Nosematidae) and Nuclear Polyhedrosis Virus Infecting the Gypsy Moth, Lymantria dispar (Lepidoptera: Lymantriidae)1
- Vectoring gypsy moth nuclear polyhedrosis virus by Apanteles melanoscelus (Hym.:Braconidae)
XML: View XML