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Source identification of western Oregon Douglas-Fir wood cores using mass spectrometry and random forest ClassificationAuthor(s): Kristen Finch; Edgard Espinoza; F. Andrew Jones; Richard Cronn
Source: Applications in Plant Sciences. 5(5): 1600158-.
Publication Series: Scientific Journal (JRNL)
Station: Pacific Northwest Research Station
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DescriptionPremise of the study: We investigated whether wood metabolite profiles from direct analysis in real time (time-of-flight) mass spectrometry (DART-TOFMS) could be used to determine the geographic origin of Douglas-fir wood cores originating from two regions in western Oregon, USA.
Methods: Three annual ring mass spectra were obtained from 188 adult Douglas-fir trees, and these were analyzed using random forest models to determine whether samples could be classified to geographic origin, growth year, or growth year and geographic origin. Specific wood molecules that contributed to geographic discrimination were identified.
Results: Douglas-fir mass spectra could be differentiated into two geographic classes with an accuracy between 70% and 76%. Classification models could not accurately classify sample mass spectra based on growth year. Thirty-two molecules were identified as key for classifying western Oregon Douglas-fir wood cores to geographic origin.
Discussion: DART-TOFMS is capable of detecting minute but regionally informative differences in wood molecules over a small geographic scale, and these differences made it possible to predict the geographic origin of Douglas-fir wood with moderate accuracy. Studies involving DART-TOFMS, alone and in combination with other technologies, will be relevant for identifying the geographic origin of illegally harvested wood.
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CitationFinch, Kristen; Espinoza, Edgard; Jones, F. Andrew; Cronn, Richard. 2017. Source identification of western Oregon Douglas-Fir wood cores using mass spectrometry and random forest Classification. Applications in Plant Sciences. 5(5): 1600158-. https://doi.org/10.3732/apps.1600158.
KeywordsDART-TOFMS, Douglas-fir, metabolites, provenance, Pseudotsuga, wood identification.
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