Skip to Main Content
U.S. Forest Service
Caring for the land and serving people

United States Department of Agriculture

Home > Search > Publication Information

  1. Share via EmailShare on FacebookShare on LinkedInShare on Twitter
    Dislike this pubLike this pub
    Author(s): D.A. ​Varley; G.K. Podila; S.T. Hiremath
    Date: 1992
    Source: Molecular and Cellular Biology. 12(10): 4539-4544.
    Publication Series: Scientific Journal (JRNL)
    Station: Northern Research Station
    PDF: Download Publication  (1.0 MB)

    Description

    Plant-pathogenic fungi produce cutinase, an enzyme required to degrade plant cuticles and facilitate penetration into the host. The absence of cutinase or a decrease in its production has been associated with a decrease in pathogenicity of the fungus. A set of isogenic strains of Cryphonectria parasitica, the chestnut blight fungus, was tested for the presence and amounts of cutinase activity. The virulent strain of C. parasitica produced and secreted significantly higher amounts of cutinase than the hypovirulent strains. Use of both nucleic acid and polyclonal antibody probes for cutinase from Fusarium solani f. sp. pisi showed that cutinase in C. parasitica is 25 kDa in size and is coded by a 1.1-kb mRNA. Both mRNA and protein were inducible by cutin hydrolysate, while hypovirlnence agents suppressed the level of mRNA and the enzyme. Since all the strains had the cutinase gene, the suppression of expression was due to the hypovirulence agents. The data presented are the first report indicating that hypovirulence agents in C. parasitica regulate a gene associated with pathogenicity in other plant-pathogenic fungi.

    Publication Notes

    • Check the Northern Research Station web site to request a printed copy of this publication.
    • Our on-line publications are scanned and captured using Adobe Acrobat.
    • During the capture process some typographical errors may occur.
    • Please contact Sharon Hobrla, shobrla@fs.fed.us if you notice any errors which make this publication unusable.
    • We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
    • This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.

    Citation

    ​Varley, D.A.; Podila, G.K.; Hiremath, S.T. 1992. Cutinase in Cryphonectria parasitica, the chestnut blight fungus: suppression of cutinase gene expression in isogenic hypovirulent strains containing double-stranded RNAs. Molecular and Cellular Biology. 12(10): 4539-4544.

    Related Search


    XML: View XML
Show More
Show Fewer
Jump to Top of Page
https://www.fs.usda.gov/treesearch/pubs/55835