Skip to Main Content
Don't make a mista(g)ke: Is tag switching an overlooked source of error in amplicon pyrosequencing studies?Author(s): Tor Carlsen; Anders Bjornsgaard Aas; Daniel Lindner; Trude Vralstad; Trond Schumacher; Havard Kauserud
Source: Fungal Ecology. 5(2012): 747-749.
Publication Series: Scientific Journal (JRNL)
Station: Northern Research Station
PDF: View PDF (165.0 KB)
DescriptionHigh throughput sequencing has become a powerful tool for fungal ecologists to explore the diversity and composition of fungal communities. However, various biases and errors are associated with the new sequencing techniques that must be handled properly. We here provide evidence for a source of error that has not yet been taken into account.
- Check the Northern Research Station web site to request a printed copy of this publication.
- Our on-line publications are scanned and captured using Adobe Acrobat.
- During the capture process some typographical errors may occur.
- Please contact Sharon Hobrla, firstname.lastname@example.org if you notice any errors which make this publication unusable.
- We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
- This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.
CitationCarlsen, Tor; Aas, Anders Bjornsgaard; Lindner, Daniel; Vralstad, Trude; Schumacher, Trond; Kauserud, Havard. 2012. Don't make a mista(g)ke: Is tag switching an overlooked source of error in amplicon pyrosequencing studies? Fungal Ecology. 5(2012): 747-749.
- Genetic characterization of uncultured fungal endophytes from Bouteloua eriopoda and Atriplex canescens
- Data mining for discovery of endophytic and epiphytic fungal diversity in short-read genomic data from deciduous trees
- Evaluation of the authenticity of a highly novel environmental sequence from boreal forest soil using ribosomal RNA secondary structure modeling
XML: View XML