Skip to Main Content
U.S. Forest Service
Caring for the land and serving people

United States Department of Agriculture

Home > Search > Publication Information

  1. Share via EmailShare on FacebookShare on LinkedInShare on Twitter
    Dislike this pubLike this pub

    Description

    Ten single-spore isolates each of Armillaria sinapina, A. cepistipes, and North American biological species (NABS)X and XI were paired in all combinations. A second set of ten single-spore isolates of each species was likewise paired. Each pairing was duplicated for a total of 3280 pairs. Using the standard morphological criteria (e.g., fluffy, crustose) to assess the pairings, A. sinapina exhibited the following compatibility levels: 68% with itself, 5.5% with A. cepistipes, 3.5% with NABS X, and 5% with NABS XI, respectively. Armillaria cepistipes was rated 53%, 6.5%, and 57% compatible with itself and NABS X and XI, respectively. NABS X was 70% self-compatible and 4.0% compatible with NABS XI. NABS XI was 55% self-compatible. The intergenic spacer region of the rDNA of each isolate was amplified using polymerase chain reaction and digested with Alu I. Seven distinct restriction fragment length polymorphic (RFLP) patterns were observed: two in A. cepistipes, two in A. sinapina, one in NABS X, one in NABS XI, and one that was common to NABS XI and A. cepistipes. By using the RFLP patterns as markers to assess the outcome of the pairings, it was determined that, in general, the morphological ratings were an accurate reflection of nuclear combination. Combining the RFLP and pairing results confirms that NABS XI is compatible with A. cepistipes and should be considered conspecific. A. sinapina, NABS X, and A. cepistipes are compatible to a very limited degree in culture, but not enough evidence exists to warrant reducing any to synonomy. Therefore, we believe NABS X should be formally described as a new species of Armillaria.

    Publication Notes

    • We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
    • This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.

    Citation

    Banik, Mark T.; Burdsall, Harold H., Jr. 1998. Assessment of compatibility among Armillaria cepistipes, A. sinapina, and North American biological species X and XI, using culture morphology and molecular biology. Mycologia. Vol. 90, no. 5 (1998).:p. 798-805.

    Keywords

    Armillaria, Compatibility, Morphology, Molecular biology

    Related Search


    XML: View XML
Show More
Show Fewer
Jump to Top of Page