Skip to Main Content
Assessing multi-scale deconstruction of wood cell wall subjected to mechanical milling for enhancing enzymatic hydrolysisAuthor(s): Jinxue Jiang; Jinwu Wang; Xiao Zhang; Michael Wolcott
Source: Industrial Crops and Products. 109: 498-508.
Publication Series: Scientific Journal (JRNL)
Station: Forest Products Laboratory
Download Publication (5.0 MB)
DescriptionThe hierarchical structure of wood cell walls resulting from complex arrangement and distribution of the heterogeneous components is considered to impact significant impediment to enzymatic hydrolysis of cellulose for biofuels. In this work, micronized wood with significant cell wall ultrastructural deconstruction were effectively produced from ring and puck milling within 12 min. In a subsequent enzymatic hydrolysis, micronized wood resulted in increase of cellulose hydrolysability by 4–14 folds over that of starting material. The underlying mechanism towards facilitating enzymatic hydrolysis was studied through delineating the ultrastructural changes and alternation of cellulose chemistry in micronized wood cell wall using SEM, TEM, CLSM, GPC, XRD, HPLC and Simon’s staining techniques. Electronic microscopy revealed distinct stages of wood cell wall deconstruction that was coincident with particle size reduction, including cell fracture and delamination, cell wall disintegration, and amorphization of cell wall fragments. Simons’ staining results also indicated increasing substrate accessibility and porosity of micronized wood, likely due to the ultrastructure alternation of cell walls. GPC and XRD revealed significant decrease of cellulose degree of polymerization (DP) and crystallinity. The correlation of these factors with cellulose hydrolysability was studied and further arranged in order through principal component analysis. The major positive factors affecting hydrolysability were surface accessibility and porosity, while cellulose crystallinity and DP were the major negative factors accompanied by particle size. The established weighed order of factors behind hydrolysability provides insights of lowering cell wall structural recalcitrance by mechanical manner.
- We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
- This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.
CitationJiang, Jinxue; Wang, Jinwu; Zhang, Xiao; Wolcott, Michael. 2017. Assessing multi-scale deconstruction of wood cell wall subjected to mechanical milling for enhancing enzymatic hydrolysis. Industrial Crops and Products. 109: 498-508.
KeywordsMechanical pretreatment, cell wall ultrastructure, cellulose structure, accessibility, enzymatic hydrolysis, principal component analysis
- Evaluation of physical structural features on influencing enzymatic hydrolysis efficiency of micronized wood
- Enzymatic hydrolysis of loblolly pine: effects of cellulose crystallinity and delignification
- Quantifying cellulose accessibility during enzyme-mediated deconstruction using 2 fluorescence-tagged carbohydrate-binding modules
XML: View XML