Skip to Main Content
Efficacy of Kamona strain Deladenus siricidicola nematodes for biological control of Sirex noctilio in North America and hybridisation with invasive conspecificsAuthor(s): Tonya D. Bittner; Nathan Havill; Isis A.L. Caetano; Ann E. Hajek
Publication Series: Scientific Journal (JRNL)
Station: Northern Research Station
Download Publication (889.0 KB)
DescriptionSirex noctilio is an invasive woodwasp that, along with its symbiotic fungus, has killed pine trees (Pinus spp.) in North America and in numerous countries in the Southern Hemisphere. We tested a biological control agent in North America that has successfully controlled S. noctilio in Oceania, South Africa, and South America. Deladenus siricidicola nematodes feed on the symbiotic white rot fungus Amylostereum areolatum and can switch to being parasitic on S. noctilio. When parasitic, the Kamona nematode strain can sterilise the eggs of S. noctilio females. However, in North America, a different strain of D. siricidicola (NA), presumably introduced along with the woodwasp, parasitises but does not sterilise S. noctilio. We tested the sterilising Kamona biological control strain of D. siricidicola against S. noctilio in North America. Interactions between the biological control strain and the NA strain could include competitive exclusion, co-infection within hosts or hybridisation. We reared D. siricidicola Kamona on an A. areolatum strain native to North America (IGS-BE) and another strain (IGS-BDF) used commercially to mass-produce the nematode in Australia. We inoculated Kamona reared on either strain of A. areolatum into logs infested with S. noctilio larvae and compared parasitism rates against control logs. Individual nematodes were isolated from S. noctilio hemocoels and from sterilised eggs and were genotyped with eight microsatellite loci. A high rate of parasitisation of S. noctilio by D. siricidicola NA was found for all treatments and we found evidence of both co-infection and hybridisation. Surprisingly, sterilisation rates were not related to the rates of parasitisation by D. siricidicola Kamona.
- Check the Northern Research Station web site to request a printed copy of this publication.
- Our on-line publications are scanned and captured using Adobe Acrobat.
- During the capture process some typographical errors may occur.
- Please contact Sharon Hobrla, firstname.lastname@example.org if you notice any errors which make this publication unusable.
- We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
- This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.
CitationBittner, Tonya D.; Havill, Nathan; Caetano, Isis A.L.; Hajek, Ann E. 2019. Efficacy of Kamona strain Deladenus siricidicola nematodes for biological control of Sirex noctilio in North America and hybridisation with invasive conspecifics. NeoBiota. 44: 39-55. https://doi.org/10.3897/neobiota.44.30402.
Keywordshybridization, coinfection, biological control, Deladenus siricidicola, Sirex noctilio, Amylostereum areolatum, parasitic nematode
- The influence of Amylostereum areolatum diversity and competitive interations on the fitness of the Sirex parasitic nematode Deladenus siricidicola
- Detection and identification of Amylostereum areolatum (Russulales: Amylostereaceae) in the mycangia of Sirex nigricornis (Hymenoptera: Siricaidae) in central Louisiana
- Intergenic spacer single nucleotide polymorphisms forg genotyping amylostereum areolatum (Russulales: Amylostereacea) symbionts of native and non-native sirex species
XML: View XML