Skip to Main Content
U.S. Forest Service
Caring for the land and serving people

United States Department of Agriculture

Home > Search > Publication Information

  1. Share via EmailShare on FacebookShare on LinkedInShare on Twitter
    Dislike this pubLike this pub
    Author(s): Taylor M. WilcoxKevin S. McKelveyMichael K. YoungCory Engkjer; Richard F. Lance; Andrew Lahr; Lisa A. Eby; Michael K. Schwartz
    Date: 2020
    Source: Environmental DNA. doi: 10.1002/edn3.80.
    Publication Series: Scientific Journal (JRNL)
    Station: Rocky Mountain Research Station
    PDF: Download Publication  (899.0 KB)


    When analyzing environmental samples for DNA from multiple taxa, researchers must usually decide between iterative analyses with single-taxon assays - which are reliable and sensitive, but also laborious to apply - and approaches such as metabarcoding that can simultaneously target multiple species, but which are less sensitive for detection across taxa. Here, we test an intermediate approach that allows efficient, parallel assessment of taxon-specific qPCR assays via high-throughput quantitative PCR (HT-qPCR). Based on an assessment of over 500 environmental samples, we found that sensitivity and specificity of our HT-qPCR approach were similar (concordance 0.900-1.000) to values achieved through single-species qPCR in six out of seven assays tested. Thus, HT-qPCR may provide analyses of similar quality as single-species qPCR analyses for environmental DNA, but at a lower cost per taxon. We see this approach as being a valuable addition to the eDNA sampling toolbox, particularly for situations where reliable inferences are needed for a defined suite of rare invasive or imperiled taxa.

    Publication Notes

    • You may send email to to request a hard copy of this publication.
    • (Please specify exactly which publication you are requesting and your mailing address.)
    • We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
    • This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.


    Wilcox, Taylor M.; McKelvey, Kevin S.; Young, Michael K.; Engkjer, Cory; Lance, Richard F.; Lahr, Andrew; Eby, Lisa A.; Schwartz, Michael K. 2020. Parallel, targeted analysis of environmental samples via high-throughput quantitative PCR. Environmental DNA. doi: 10.1002/edn3.80.


    Google Scholar


    environmental DNA, multi-species, multi-taxa, qPCR, rare species

    Related Search

    XML: View XML
Show More
Show Fewer
Jump to Top of Page