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Metabarcoding for Phytophthora—benefits and limitationsAuthor(s): Neelam Redekar; Joyce Eberhart; Ebba Peterson; Jennifer Parke
Source: Proceedings of the seventh sudden oak death science and management symposium: healthy plants in a world with <em>Phytophthora</em>. Gen. Tech. Rep. PSW-GTR-268
Publication Series: General Technical Report (GTR)
Station: Pacific Southwest Research Station
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DescriptionThe metabarcoding approach has revolutionized the study of microbial ecology with its ability to detect microbial DNA at an unprecedented depth and coverage. This method exploits highthroughput sequencing and DNA barcoding for identification of microbial species, including novel and uncultivable microbes. The metabarcoding approach is gaining more interest by the Phytophthora scientific community for monitoring the existence and spread of Phytophthora species in diverse habitats. Understanding and mitigating metabarcoding limitations is crucial for achieving better resolution and accurate determination of the diversity of Phytophthora species.
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CitationRedekar, Neelam; Eberhart, Joyce; Peterson, Ebba; Parke, Jennifer. 2020. Metabarcoding for Phytophthora—benefits and limitations. In: Frankel, Susan J.; Alexander, Janice M., tech. cords. Proceedings of the seventh sudden oak death science and management symposium: healthy plants in a world with Phytophthora. Gen. Tech. Rep. PSW-GTR-268. Albany, CA: U.S. Department of Agriculture, Forest Service, Pacific Southwest Research Station: 50-53.
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