Abstract
The metabarcoding approach has revolutionized the study of microbial ecology with its ability to detect microbial DNA at an unprecedented depth and coverage. This method exploits highthroughput sequencing and DNA barcoding for identification of microbial species, including novel and uncultivable microbes. The metabarcoding approach is gaining more interest by the
Phytophthora scientific community for monitoring the existence and spread of
Phytophthora species in diverse habitats. Understanding and mitigating metabarcoding limitations is crucial for achieving better resolution and accurate determination of the diversity of
Phytophthora species.
Parent Publication
Citation
Redekar, Neelam; Eberhart, Joyce; Peterson, Ebba; Parke, Jennifer. 2020. Metabarcoding for
Phytophthora—benefits and limitations. In: Frankel, Susan J.; Alexander, Janice M., tech. cords. Proceedings of the seventh sudden oak death science and management symposium: healthy plants in a world with
Phytophthora. Gen. Tech. Rep. PSW-GTR-268. Albany, CA: U.S. Department of Agriculture, Forest Service, Pacific Southwest Research Station: 50-53.