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Rapid recovery and detection of Phytophthora ramorum propagules in nursery water

Author(s):

Douglas Luster
Karen Suslow
Supriya Sharma
Wolfgang Schweigkofler
Vernon Huffman

Year:

2020

Publication type:

General Technical Report (GTR)

Primary Station(s):

Pacific Southwest Research Station

Source:

Proceedings of the seventh sudden oak death science and management symposium: healthy plants in a world with <em>Phytophthora</em>. Gen. Tech. Rep. PSW-GTR-268

Description

Phytophthora ramorum, causal agent of sudden oak death, continues to threaten U.S. forest ecosystems and the nursery industry. Currently, USDA APHIS’s protocol (2014) utilizes the Bottle of Bait (BOB) recovery method for P. ramorum, which requires collecting water from a source, baiting with healthy rhododendron leaves for a 3-day incubation period, followed by plating on semi-selective media. Rapid methods are needed for recovery and detection of P. ramorum propagules from water sources. Working at the National Ornamentals Research Site at Dominican University of California (NORS-DUC), we are developing rapid water filtration and flocculation methods for recovery and detection of P. ramorum propagules from nursery irrigation water. A mock irrigation pond was established with flow from a P. ramorum- infested plot into an adjoining plot. Antibodies raised against P. ramorum -specific secreted proteins were applied for detection of zoospores and sporangia from 1 L samples in filter extracts or alum flocculates using standard immunoassay procedures. Results with spiked samples indicate that propagules of P. ramorum recovered by filtration or flocculation from spiked nursery water samples can be detected in 24 h or less.

Citation

Luster, Douglas; Suslow, Karen; Sharma, Supriya; Schweigkofler, Wolfgang; Huffman, Vernon. 2020. Rapid recovery and detection of Phytophthora ramorum propagules in nursery water. In: Frankel, Susan J.; Alexander, Janice M., tech. cords. Proceedings of the seventh sudden oak death science and management symposium: healthy plants in a world with Phytophthora. Gen. Tech. Rep. PSW-GTR-268. Albany, CA: U.S. Department of Agriculture, Forest Service, Pacific Southwest Research Station: 83-90.

Publication Notes

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https://www.fs.usda.gov/treesearch/pubs/60509