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Using metagenomics to quantify the bacterial diversity in field stakes treated with wood preservativeAuthor(s): Lakshmi Narayanan; Juliet D. Tang; Grant Kirker; Maryam K. Mohammadi-Araghi; C. Elizabeth. Stokes
Source: In: Proceedings, 62nd International Convention of Society of Wood Science and Technology. 111-118.
Publication Series: Paper (invited, offered, keynote)
Station: Forest Products Laboratory
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DescriptionWood is one of the primary construction materials used in the United States. However, it is susceptible to decay over time, leading to its reduced service life. Development of targeted wood protection methods requires detailed understanding of microbial wood-decay mechanisms. Fungi are the most destructive microorganisms to attack wood and much research effort has been directed towards understanding their mechanisms of decay. The role of bacteria in wood-decay is less studied. The objective of this research was to compare bacterial communities colonizing pressure-treated and untreated wooden field stakes using next-generation sequencing technology at two locations (Saucier, MS and Madison, WI). Micronized copper azole (MCA), copper azole type C (CA-C), chromated copper arsenate (CCA), and untreated (UN) wooden field stakes of size ¾”H x ¾”W x 16”L (19 mm x 19 mm x 40.6 cm) were installed at field test sites in Saucier, MS and Madison, WI. Stakes were collected at three different time points within a year of installation. DNA was extracted from sawdust drilled from the A-horizon (within 7.6 cm of the soil line) of the field stakes and then used for 16S metagenomics library preparation. Metagenomic libraries were sequenced using the Illumina MiSeq platform. Data analysis from the three-month collection period revealed sufficient sampling depth and high species diversity. Principal components analysis indicated that the bacterial communities colonizing the CA-C, MCA, and UN field stakes were distinctly different. In addition, the CCA stakes showed some stake-dependent similarities with some MCA stakes. These trends were observed at both field sites. Furthermore, PCA loadings plots showed that the species driving these differences were not the same across two sites. One minor exception was Luteibacter rhizovicinus. The latter species was an influencing factor driving the community similarity among some CCA and UN stakes (MS) or some CCA stakes and one MCA stake (MS).
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CitationNarayanan, Lakshmi; Tang, Juliet D.; Kirker, Grant; Mohammadi-Araghi, Maryam K.; Stokes, C. Elizabeth. 2019. Using metagenomics to quantify the bacterial diversity in field stakes treated with wood preservative. In: Proceedings, 62nd International Convention of Society of Wood Science and Technology. 111-118.
KeywordsPressure-treated wood, wood preservative, metagenomics, bacterial community analysis
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