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Omics analyses and biochemical study of Phlebiopsis gigantea elucidate its degradation strategy of wood extractives

Author(s):

Mana Iwata
Ana Gutiérrez
Gisela Marques
Grzegorz Sabat
Jennifer M. Bhatnagar
Jagjit Yadav
Anna Lipzen
Yuko Yoshinaga
Aditi Sharma
Catherine Adam
Christopher Daum
Vivian Ng
Igor V Grigoriev
Chiaki Hori

Year:

2021

Publication type:

Scientific Journal (JRNL)

Primary Station(s):

Forest Products Laboratory

Source:

Scientific Reports

Description

Wood extractives, solvent-soluble fractions of woody biomass, are considered to be a factor impeding or excluding fungal colonization on the freshly harvested conifers. Among wood decay fungi, the basidiomycete Phlebiopsis gigantea has evolved a unique enzyme system to efciently transform or degrade conifer extractives but little is known about the mechanism(s). In this study, to clarify the mechanism(s) of softwood degradation, we examined the transcriptome, proteome, and metabolome of P. gigantea when grown on defned media containing microcrystalline cellulose and pine sapwood extractives. Beyond the conventional enzymes often associated with cellulose, hemicellulose and lignin degradation, an array of enzymes implicated in the metabolism of softwood lipophilic extractives such as fatty and resin acids, steroids and glycerides was signifcantly up-regulated. Among these, a highly expressed and inducible lipase is likely responsible for lipophilic extractive degradation, based on its extracellular location and our characterization of the recombinant enzyme. Our results provide insight into physiological roles of extractives in the interaction between wood and fungi.

Citation

Iwata, Mana; Gutiérrez, Ana; Marques, Gisela; Sabat, Grzegorz; Kersten, Philip J.; Cullen, Daniel; Bhatnagar, Jennifer M.; Yadav, Jagjit; Lipzen, Anna; Yoshinaga, Yuko; Sharma, Aditi; Adam, Catherine; Daum, Christopher; Ng, Vivian; Grigoriev, Igor V.; Hori, Chiaki. 2021. Omics analyses and biochemical study of Phlebiopsis gigantea elucidate its degradation strategy of wood extractives. Scientific Reports. 11(1): 12528. https://doi.org/10.1038/s41598-021-91756-5.

Cited

Publication Notes

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  • This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.
https://www.fs.usda.gov/treesearch/pubs/62692