Skip to Main Content
Phylogenetic Relationships in Actinidia as Revealed by RAPD AnalysisAuthor(s): Hongwen Huang; Zuozhou Li; Jianqiang Li; Thomas L. Kubiisiak; Desmond R. Lavne
Source: J. Amer. Soc Hort. Sci. 127(5):759-766. 2002.
Publication Series: Miscellaneous Publication
PDF: View PDF (627 KB)
DescriptionPhylogenetic relationships within the Actinidia were investigated using randomly amplified polymorphic DNA (RAPD) markers. DNAs from 10 taxa, including31 species encompassing all four sections and four series of the traditional subdivisions within the genus, were amplified using 22 preselected 10-mer oligonucieotide primers. A total 204 DNA bands were scored across the 10 taxa, of which 188 (92%) were polymorphic. A wide range of genetic similarity was observed among the taxa (0.13 to 0.61). The average similarity between varieties of the same species was 0.54, and between different species was 0.28. respectively. Although the phylogenetic analysis revealed a clear indication that section Leiocarpae was a monophyletic group, subdivisions of the other three traditional sections were poorly supported. The UPGMA phenogram showed that the majority of the species clustered into geographic subgroups in accordance with their natural distribution (the Yangtzi River, southeastern China, southern China and southwestern China). The intrageneric subdivisions of Actinidia appeared to be difficult, but some subdivisions could be explained by the geographic distribution of the species, particularly for species of Gang's sections of Maculatae and Stellatae. The phylogenetic relationships among several species with previous taxonomic uncertainty are also discussed on the basis of the RAPD data. The results of this study supplement our previous understanding of the Actinidia taxonomy based solely on morphological characters.
- You may send email to firstname.lastname@example.org to request a hard copy of this publication.
- (Please specify exactly which publication you are requesting and your mailing address.)
- We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
- This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.
CitationHuang, Hongwen; Li, Zuozhou; Li, Jianqiang; Kubiisiak, Thomas L.; Lavne, Desmond R. 2002. Phylogenetic Relationships in Actinidia as Revealed by RAPD Analysis. J. Amer. Soc Hort. Sci. 127(5):759-766. 2002.
Keywordsintrageneric subdivision, geographic groups, kiwifruit
- Selection of RAPD markers for investigation of genetic population structure in fusiform rust fungus infecting loblolly pine
- Geographic variation, genetic structure, and conservation unit designation in the Larch Mountain salamander (Plethodon larselli).
- A molecular phylogeny for Cercocarpus H.B.K. (Rosaceae) using the external transcribed spacer of the nuclear ribosomal repeat
XML: View XML