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Significant levels of extracellular reactive oxygen species produced by brown rot basidiomycetes on celluloseAuthor(s): Roni Cohen; Kenneth A. Jensen; Carl J. Houtman; Kenneth E. Hammel
Source: FERS letters. Vol. 531 (2002): Pages 483-488
Publication Series: Miscellaneous Publication
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DescriptionIt is often proposed that brown rot basidiomycetes use extracellular reactive oxygen species (ROS) to accomplish the initial depolymerization of cellulose in wood, but little evidence has been presented to show that the fungi produce these oxidants in physiologically relevant quantities. We used [14C]phenethyl polyacrylate as a radical trap to estimate extracellular ROS production by two brown rot fungi, Gloeophyllum trabeum and Postia placenta, that were degrading cellulose. Both fungi oxidized aromatic rings on the trap to give monohydroxylated and more polar products in significant yields. All of the cultures contained 2,5-dimethoxyhydroquinone, a fungal metabolite that has been shown to drive Fenton chemistry in vitro. These results show that extracellular ROS occur at significant levels in cellulose colonized by brown rot fungi, and suggest that hydroquinone-drivenROS production may contribute to decay by diverse brown rot species.
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CitationCohen, Roni; Jensen, Kenneth A.; Houtman, Carl J.; Hammel, Kenneth E. 2002. Significant levels of extracellular reactive oxygen species produced by brown rot basidiomycetes on cellulose. FERS letters. Vol. 531 (2002): Pages 483-488
KeywordsWood decay, hydroxyl radical, aromatic hydroxylation, 2, 5-Dimethoxyhydroquinone, Gloeophyllum trabeum: Postia placenta
- Evidence from Serpula lacrymans that 2,5-Dimethoxyhydroquinone Is a Lignocellulolytic Agent of Divergent Brown Rot Basidiomycetes
- Diversity of lignicolous basidiomycetes in coarse woody debris
- Evidence for cleavage of lignin by a brown rot basidiomycete
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