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    Description

    High-throughput screening requires simple assays that give reliable quantitative results. A microplate assay was developed for reducing sugar analysis that uses a 2,2'-bicinchoninic-based protein reagent. Endo-1,4-â-D-xylanase activity against oat spelt xylan was detected at activities of 0.002 to 0.011 IU ml−1. The assay is linear for sugar concentrations from 0 to 86 µg ml−1 and can also be used to assay protein concentrations (0 to 143 µg ml−1) on the same plate. A variety of temperatures and pH conditions can be used and, after incubation, the assay requires only one detection reagent and one heating step.

    Publication Notes

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    • This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.

    Citation

    Kenealy, William R.; Jeffries, Thomas W. 2003. Rapid 2,2''-bicinchoninic-based xylanase assay compatible with high throughput screening. Biotechnology letters. Vol. 25 (2003): Pages 1619-1623

    Keywords

    Bicinchoninic acid, high throughput, xylanase

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https://www.fs.usda.gov/treesearch/pubs/9184