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Comparison of high performance liquid chromatography and enzymatic analysis of soluble carbohydrates in loblolly pine
Foliar tissue was collected from a field study designed to test impacts of atmospheric pollutants on loblolIy pine (
Pinus taeda
L.) seedlings. Standard enzymatic (ENZ) and high performance liquid chromatography (HPLC) methods were used to analyze the tissue for soluble sugars. A comparison of the methods revealed no significant diffennces in accuracy...
https://www.fs.usda.gov/treesearch/search?keywords=%22Liquid+chromatography%22
Author(s):
Patricia L. Faulkner; Michele M. Schoeneberger; Kim H. Ludovici
Year:
1993
Keywords:
Source:
Community Soil Scientist Plant Analysis, 24(1&2), 149-160(1993)
Determination of Picloram in Soil and Water by Reversed-Phase Liquid Chromatography
A reversed-phase liquid chromatographic method is presneted for the determination of picloram in the parts per billion (ppb) range in soil, soil solution, and stream samples. Quanitification is effected by UV absorpation at 254 nm. Derivatization is not necessary. The method permits 92% ± 7.1 recovery from water samples and 61.8% ± 11.1 recovery from soil samples....
https://www.fs.usda.gov/treesearch/search?keywords=%22Liquid+chromatography%22
Author(s):
M.J.M. Wells; J.L. Michael; D.G. Neary
Year:
1984
Keywords:
Source:
Arch. Environ. Contam. Toxicol. 13. 234-235 (1984)
Separation and quantification of monothiols and phytochelatins from a wide variety of cell cultures and tissues of trees and other plants using high performance liquid chromatography
The HPLC method presented here for the quantification of metal-binding thiols is considerably shorter than most previously published methods. It is a sensitive and highly reproducible method that separates monobromobimane tagged monothiols (cysteine, glutathione, γ-glutamylcysteine) along with polythiols (PC
2
, PC
3
...
https://www.fs.usda.gov/treesearch/search?keywords=%22Liquid+chromatography%22
Author(s):
Rakesh Minocha; P. Thangavel; Om Parkash Dhankher; Stephanie Long
Year:
2008
Keywords:
algae, Arabidopsis, Crambe, forest trees, HPLC, mBBr, phytochelatins, sugar maple, red spruce, rice
Source:
Journal of Chromatography A. 1207: 72-83.
Thermospray ionization liquid chromatography-mass spectrometry and chemical ionization gas chromatography- mass spectrometry of hexazinone metabolites in soil and vegetation extracts
We have used thermospray LC-MS to confirm three highly polar metabolites (A, B. and G) of the herbicide hexazinone [3-cyclohexyl-6-(dimethylamino)-l-methyl-1,3,5-triazine-2,4(1H,3H)-dione]. and chemical ionization GC-MS to confirm two other metabolites (D and E) in extracts of soil and vegetation from a forest in the Central Alabama Piedmont. Selected-ion monitoring (...
https://www.fs.usda.gov/treesearch/search?keywords=%22Liquid+chromatography%22
Author(s):
Joseph B. Fischer; Jerry L. Michael
Year:
1995
Keywords:
Source:
Journal of Chromatography A. 704: 131-139
Simultaneous determination of UV-filters and estrogens in aquatic invertebrates by modified quick, easy, cheap, effective, rugged, and safe extraction and liquid chromatography tandem mass spectrometry
tUltraviolet-filters (UV-filters) and estrogens have attracted increased attention as contaminants of emerging concern (CECs) due to their widespread occurrence in the environment. Most of these CECs are hydrophobic and have the potential to accumulate in aquatic organisms. To date, co-analysis of UV-filters and estrogens has not been reported due, in part, to the...
https://www.fs.usda.gov/treesearch/search?keywords=%22Liquid+chromatography%22
Author(s):
Ke He; Anne Timm; Lee Blaney
Year:
2017
Keywords:
QuEChERS, Reverse-solid-phase, extraction, Wrong-way-round ionization, UV-filters, Estrogens, Invertebrates
Source:
Journal of Chromatography A
Simultaneous separation and quantitation of amino acids and polyamines of forest tree tissues and cell cultures within a single high-performance liquid chromatography run using dansyl derivatization
The objective of the present study was to develop a rapid HPLC method for simultaneous separation and quantitation of dansylated amino acids and common polyamines in the same matrix for analyzing forest tree tissues and cell cultures. The major modifications incorporated into this method as compared to previously published HPLC methods for separation of only dansyl...
https://www.fs.usda.gov/treesearch/search?keywords=%22Liquid+chromatography%22
Author(s):
Rakesh Minocha; Stephanie Long
Year:
2004
Keywords:
Derivatization, LC, Amino acids, Dns derivatives, Polyamines, Amines, Putrescine, Spermidine, Spermine
Source:
Journal of Chromatography A. 1035: 63-73.
Time- and cost-saving apparatus for analytical sample filtration
Simple and cost-effective protocols were developed for removing particulates from samples prior to analysis by high performance liquid chromatography and gas chromatography. A filter and vial holder were developed for use with a 96-well filtration plate. The device saves preparation time and costs.
https://www.fs.usda.gov/treesearch/search?keywords=%22Liquid+chromatography%22
Author(s):
William R. Kenealy; Joseph C. Destree
Year:
2005
Keywords:
Analytical preparation, filtration
Source:
Research Note FPL-RN-0298. Madison, WI: U.S. Department of Agriculture, Forest Service, Forest Products Laboratory. 2 p.
A Gas-Liquid Chromatographic Examination of Stilbene Derivatives
Trimethylsilyl ether derivatives of twenty hydroxystilbenes were separated by gas-liquid chromatography on Apiezon-L, OV
-1
, OV
-17
, and SE
-54
. Relative retention times were highly dependent upon the degree of hydroxylation and methoxylation, the positions of these groups and on
cis-trans
...
https://www.fs.usda.gov/treesearch/search?keywords=%22Liquid+chromatography%22
Author(s):
Richard W. Hemingway; W. E. Hillis; K Burerton
Year:
1970
Keywords:
Source:
Journal of Chromatography 50(3):391-399
Kinetics on cocondensation between phenol and urea through formaldehyde I
The kinetics of the reactions of methylolphenols and urea were investigated using 2- and 4-hydroxybenzyl alcohols. The high-performance liquid chromatography (HPLC) using a reverse-phase column gave a clear separation between methylolphenols and hydroxybenzylureas. The molar ratios of hydroxybenzylureas to the corresponding methylolphenols in reaction mixtures were...
https://www.fs.usda.gov/treesearch/search?keywords=%22Liquid+chromatography%22
Author(s):
Yasunori Yoshida; Bunchiro Tomita; Chung-Yun Hse
Year:
1995
Keywords:
Source:
Mokuzai Gakkaishi 41(6):547-554
Rapid analysis of abietanes in conifers
Diterpene resin acids are major constituents of conifer oleoresin and play important roles in tree defense against insects and microbial pathogens. The tricyclic C-20 carboxylic acids are generally classified into two groups, the abietanes and the pimaranes. The abietanes have conjugated double bonds and exhibit characteristic UV spectra. Here, we report the analysis...
https://www.fs.usda.gov/treesearch/search?keywords=%22Liquid+chromatography%22
Author(s):
P.J. Kersten; B.J. Kopper; K.F. Raffa; B.L. Illman
Year:
2006
Keywords:
Abietic, neoabietic, palustric, levopimaric, dehydroabietic, white spruce, diterpenes, HPLC, abietic acid, high performance liquid chromatography, oleoresins, carboxylic acids, dehydroabietic acid, neoabietic acid, palustric acid, pimaranes, abietanes, resin acids, levopimaric acid
Source:
Journal of chemical ecology. Vol. 32 (2006): pages 2679-2685.
Extraction and estimation of the quantity of calcium oxalate crystals in the foliage of conifer and hardwood trees
The main goal of this study was to develop a method for the extraction and indirect estimation of the quantity of calcium oxalate (CaOx) in the foliage of trees. Foliar tissue was collected from a single tree of each species (five conifers and five hardwoods) for comparison of extractions in different solvents using 10 replicates per species from the same pool of...
https://www.fs.usda.gov/treesearch/search?keywords=%22Liquid+chromatography%22
Author(s):
Rakesh Minocha; Bradley Chamberlain; Stephanie Long; Swathi A. Turlapati; Gloria Quigley
Year:
2015
Keywords:
aspen, beech, birch, conifers, fir, hardwoods, hemlock, HPLC, maple, oak, organic acids, pine, spruce
Source:
Tree Physiology. 35(5): 574-580.
Comparative transcriptome and secretome analysis of wood decay fungi Postia placenta and Phanerochaete chrysosporium
Cellulose degradation by brown rot fungi, such as Postia placenta, is poorly understood relative to the phylogenetically related white rot basidiomycete, Phanerochaete chrysosporium. To elucidate the number, structure, and regulation of genes involved in lignocellulosic cell wall attack, secretome and transcriptome analyses were performed on both wood decay fungi...
https://www.fs.usda.gov/treesearch/search?keywords=%22Liquid+chromatography%22
Author(s):
Amber J. Vanden Wymelenberg; Jill Gaskell; Michael Mozuch; Grzegorz Sabat; John Ralph; Oleksandr Skyba; Shawn D Mansfield; Robert A. Blanchette; Diego Martinez; Igor Grigoriev; Philip J Kersten; Daniel Cullen
Year:
2010
Keywords:
Liquid chromatography, cellulose, biodegradation, mass spectrometry, enzymes, biotechnology, lignocellulose, Basidiomycetes, molecular genetics, fungi genetics, fungi, lignin, biodegradation, wood-decaying fungi, industrial applications, gene expression, genetic transcription, Postia placenta, Phanerochaete chrysosporium, brown rot, aspen, glucose, iron, quinone, chemical reactions, Populus, transcriptome, wood decay, secretome, white rot, decay fungi, Fenton system
Source:
Applied and environmental microbiology. Vol. 76, no. 11 (June 2010): p. 3599-3610.
Influence of Populus Genotype on Gene Expression by the Wood Decay Fungus Phanerochaete chrysosporium
We examined gene expression patterns in the lignin-degrading fungus Phanerochaete chrysosporium when it colonizes hybrid poplar (Populus alba tremula) and syringyl (S)-rich transgenic derivatives. Acombination ofmicroarrays and liquid chromatography- tandem mass spectrometry (LC-MS/MS) allowed detection of a total of 9,959 transcripts and 793...
https://www.fs.usda.gov/treesearch/search?keywords=%22Liquid+chromatography%22
Author(s):
Jill Gaskell; Amber Marty; Michael Mozuch; Philip J. Kersten; Sandra Splinter Bondurant; Grzegorz Sabat; Ali Azarpira; John Ralph; Oleksandr Skyba; Shawn D. Mansfield; Robert A. Blanchette; Dan Cullen
Year:
2014
Keywords:
Phanerochaete, lignin, Populus, transgenic, transcriptome, secretome
Source:
Appl. Environ. Microbiol. 2014, Volume 80, Number 18, 2014; pp. 5828-5835.
Temporal Alterations in the Secretome of the Selective Ligninolytic Fungus
Ceriporipsis subvermispora
during growth on Aspen Wood Reveal this Organism's Strategy for Degrading Lighnocellulose
The white-rot basidiomycetes efficiently degrade all wood cell wall polymers. Generally, these fungi simultaneously degrade cellulose and lignin, but certain organisms, such as
Ceriporiopsis subvermispora
, selectively remove lignin in advance of cellulose degradation. However, relatively little is known about themechanismof selective ligninolysis. To...
https://www.fs.usda.gov/treesearch/search?keywords=%22Liquid+chromatography%22
Author(s):
Chiaki Hori; Jill Gaskell; Kiyohiko Igarashi; Phil Kersten; Michael Mozuch; Masahiro Samejima; Dan Cullen
Year:
2014
Keywords:
Ceriporiopsis, lignin, secretome, cellulose, hemicellulose
Source:
Appl. Environ. Microbiol. 2014, Volume 80, Number 7, pp. 2062-2070.
Surface Enrichment by Conventional and Polymerizable Sulfated Nonylphenol Ethoxylate Emulsifiers in Water-Based Pressure-Sensitive Adhesive
Comparisons of properties are made for pressure-sensitive adhesives (PSAs) generated via emulsion polymerization using both conventional and reactive emulsifiers. The emulsifiers are ammonium salts of sulfated nonylphenol ethoxylates with similar chemical structures and hydrophilic−lipophilic balances. The polymerizable surfactant possesses a reactive double...
https://www.fs.usda.gov/treesearch/search?keywords=%22Liquid+chromatography%22
Author(s):
Jilin Zhang; Yuxi Zhao; Matthew R. Dubay; Steven J. Severtson; Larry E. Gwin; Carl J. Houtman
Year:
2013
Keywords:
pressure sensitive adhesive, surfactant, migration
Source:
Ind. Eng. Chem. Res. Volume 52, 2013; pp. 8616-8621.
Transcriptome and secretome analyses of Phanerochaete chrysosporium reveal complex patterns of gene expression
The wood decay basidiomycete Phanerochaete chrysosporium was grown under standard ligninolytic or cellulolytic conditions and subjected to whole-genome expression microarray analysis and liquid chromatography-tandem mass spectrometry of extracellular proteins. A total of 545 genes were flagged on the basis of significant changes in transcript accumulation and/or...
https://www.fs.usda.gov/treesearch/search?keywords=%22Liquid+chromatography%22
Author(s):
Amber J. Vanden Wymelenberg; Jill A. Gaskell; Michael D. Mozuch; Philip J. Kersten; Grzegorz Sabat; Diego Martinez; Daniel Cullen
Year:
2009
Keywords:
Liquid chromatography, cellulose, peptides, wood biodegradation, biodegradation, genomes, mass spectrometry, enzymes, biotechnology, lignocellulose, proteins, Basidiomycetes, molecular genetics, fungi, genetics, lignin, wood-decaying fungi, industrial applications, gene expression, manganese peroxidase, transcriptome, wood decay, secretome, Phanerochaete chrysosporium, white rot
Source:
Applied and environmental microbiology. Vol. 75, no. 12 (June 2009): pages 4058-4068.
Formaldehyde condensation products of model phenols for conifer bark tannins
Gel permeation chromatograpy of the condensation products of phenols and formaldehyde proved effective in understanding the reactions of condensed tannins with formaldehyde. Rates of condensation of phloroglucinols, resorcinols, catechols, (+)catechin, and (-)epicatechin were examined to determine if methylol-tannins from southern pine bark could be prepared as resin...
https://www.fs.usda.gov/treesearch/search?keywords=%22Liquid+chromatography%22
Author(s):
R.W. Hemingway; G.W. McGraw
Year:
1978
Keywords:
Source:
Journal of Liquid Chromatography 1(2):163-179
Antifungal metabolites of lactobacilli
In recent years, public concern about indoor mold growth has increased dramatically in the United States. In this study, lactic acid bacteria, which are known to produce antimicrobial compounds important in the biopreservation of food, were evaluated to determine if the same antimicrobial properties can be used to protect wood from mold establishment. Based on biomass...
https://www.fs.usda.gov/treesearch/search?keywords=%22Liquid+chromatography%22
Author(s):
Vina A. Yang; Carol A. Clausen
Year:
2004
Keywords:
Lactobacilli, antifungal metabolites, mold, lactic acid bacteria, metabolites, antimicrobial compounds, growth inhibition, bacteria
Source:
Proceedings from the Woodframe Housing Durability and Disaster Issues Conference, October 4-6, 2004 ... Las Vegas, Nevada, USA. Madison, WI : Forest Products Society, [2004]: p. 307-311.
Environmental Fate and Impacts of Sulfometuron on Watersheds in the Southern United States
Dissipation of sulfometuron (SM), methyl 2-[[[[(4,6-diiethyl-2-pyrimidinyl)amino]carbonyl]amino]sulfonyl] benzoate, in streamflow, sediment, plant tissue, litter, and soil following operational forestry applications at the target rate of 0.42 kg a.i. ha
-1
was monitored. Streamflow samples were collected at a weir on the perimeter and 30...
https://www.fs.usda.gov/treesearch/search?keywords=%22Liquid+chromatography%22
Author(s):
J.L. Michael
Year:
2003
Keywords:
Source:
J. Environ. Qual. 32:456-465 (2003).
Isolation and purification of pyranose 2-oxidase from Phanerochaete chrysosporium and characterization of gene structure and regulation
Pyranose 2-oxidase (POX) was recovered from Phanerochaete chrysosporium BKM-F-1767 solid substrate culture using mild extraction conditions and was purified. 13C-nuclear magnetic resonance confirmed production of D- arabino -hexos-2-ulose (glucosone) from D-glucose with the oxidase. Peptide fingerprints generated by liquid chromatography-tandem mass spectrometry of...
https://www.fs.usda.gov/treesearch/search?keywords=%22Liquid+chromatography%22
Author(s):
Theodorus H. de Koker; Michael D. Mozuch; Daniel Cullen; Jill Gaskell; Philip J. Kersten
Year:
2004
Keywords:
Phanerochaete chrysosporium, pyranose 2-oxidase, gene structure, gene regulation, POX, isolation, purification
Source:
Applied and environmental microbiology. Vol. 70, no. 10 (Oct. 2004): Pages 5794-5800
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