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  1. Extracellular oxidative systems of the lignin-degrading Basidiomycete Phanerochaete chrysosporium

    Author(s):
    Phil Kersten; Dan Cullen
    Year:
    2007
    Keywords:
    Source:

  2. Phanerochaete chrysosporium genomics

    A high quality draft genome sequence has been generated for the lignocellulose-degrading basidiomycete Phanerochaete chrysosporium (Martinez et al. 2004). Analysis of the genome in the context of previously established genetics and physiology is presented. Transposable elements and their potential relationship to genes involved in lignin degradation are systematically...

    Author(s):
    Luis F. Larrondo; Rafael Vicuna; Dan Cullen
    Year:
    2005
    Keywords:
    Enzymes, lignocellulose, genetic transcription, fungi, industrial applications, Basidiomycetes, wood-decaying fungi, biodegradation, genomes, nucleotide sequence, molecular genetics, Phanerochaete chrysosporium, white rot, wood decay
    Source:
    Applied mycology and biotechnology. Volume 5, Genes and genomics. Amsterdam : Boston : Elsevier, 2005: ISBN: 0444518088: 9780444518088: p. [315]-352.

  3. The nop gene from Phanerochaete chrysosporium encodes a peroxidase with novel structural features

    Inspection of the genome of the ligninolytic basidiomycete Phanerochaete chrysosporium revealed an unusual peroxidase-like sequence. The corresponding full length cDNA was sequenced and an archetypal secretion signal predicted. The deduced mature protein (NoP, novel peroxidase) contains 295 aa residues and is therefore considerably shorter than other Class II (fungal)...

    Author(s):
    Luis F. Larrondo; Angel Gonzalez; Tomas Perez-Acle; Dan Cullen; Rafael Vicuna
    Year:
    2005
    Keywords:
    Oxidoreductase, peroxidase, Phanerochaete chrysosporium, modeling, molecular dynamics
    Source:
    Biophysical chemistry. Vol. 116 (2005): pages 167-173

  4. Identification of phanerosporic acid in birch degraded by Phanerochaete chrysosporium

    Extracts of Phanerochaete chrysosporium cultures grown on birch or on a malt extract-peptone-glucose agar medium were analysed by HPLC. A major component from the two sources appears to be identical by HPLC and UV- visible spectrometry. The product isolated from agar-grown cultures was purified to apparent homogeneity and structure analysis by NMR indicates that the...

    Author(s):
    Michael D. Mozuch; Philip J. Kersten
    Year:
    2003
    Keywords:
    Phanerochaete chrysosporium, resorcylate, betulachrysoquinone, phanerosporic acid, wood-decaying fungi, yellow birch, Basidiomycetes, high performance liquid chromatography, nuclear magnetic resonance, fungal metabolites, cultures and culture media
    Source:
    Thirty-fourth annual meeting of the International Research Group on Wood Preservation : 2003 May 18-23, Brisbane, Queensland, Australia. Stockholm, Sweden : IRG Secretariat, 2003: 5 pages.

  5. Organization and differential regulation of a cluster of lignin peroxidase genes of Phanerochaete chrysosporium

    The lignin peroxidases of Phanerochaete chrysosporium are encoded by a minimum of 10 closely related genes. Physical and genetic mapping of a cluster of eight lip genes revealed six genes occurring in pairs and transcriptionally convergent, suggesting that portions of the lip family arose by gene duplication events. The completed sequence of 1ipG and lipJ, together...

    Author(s):
    Philip Stewart; Daniel Cullen
    Year:
    1999
    Keywords:
    Phanerochaete chrysosporium, Wood destroying fungi, Decay fungi, Lignin, Peroxidases, Genetic mapping, Genetic regulation
    Source:
    Journal of bacteriology. Vol. 181 (June 1999).:p. 3427-3432 : ill.

  6. Genomic organization of a cellulase gene family in Phanerochaete chrysosporium

    Southern blot and nucleotide sequence analysis of Phanerochaete chrysosporium BKM-F-1767 genomic clones indicate that this wood-degrading fungus contains at least six genes with significant homology to the Trichoderma reesei cellobiohydrolase I gene (cbh1). Using pulsed-field gel electrophoresis to separate P. chrysosporium chromosomes, the six cellulase genes were...

    Author(s):
    Sarah F. Covert; Jennifer Bolduc; Daniel Cullen
    Year:
    1992
    Keywords:
    Molecular genetics, fungi, genetics, wood-decaying fungi, biodegradation, fungi, biotechnology, Basidiomycetes, nucleotide sequence, gene mapping, cellulase, genomes, cellulose 1, 4-beta-cellobiosidase, lignocellulose, gel electrophoresis, Phanerochaete chrysosporium, genetic engineering, wood decay, white rot, Trichoderma reesei, pulsed-field gel electrophoresis, southern blot analysis
    Source:
    Current genetics. Vol. 22 (1992): p. 407-413. Current genetics. Vol. 23 (1993): p. 374 (erratum).

  7. Structure, organization, and transcriptional regulation of a family of copper radical oxidase genes in the lignin-degrading basidiomycete Phanerochaete chrysosporium

    The white rot basidiomycete Phanerochaete chrysosporium produces an array of nonspecific extracellular enzymes thought to be involved in lignin degradation, including lignin peroxidases, manganese peroxidases, and the H2O2-generating copper radical oxidase, glyoxal oxidase (GLX). Preliminary analysis of the P. chrysosporium draft genome had identified six sequences...

    Author(s):
    Amber Vanden Wymelenberg; Grzegorz Sabat; Michael Mozuch; Philip J. Kersten; Dan Cullen; Robert A. Blanchette
    Year:
    2006
    Keywords:
    Liquid chromatography, enzymes, mass spectrometry, peroxidases, Basidiomycetes, lignin, biodegradation, wood-decaying fungi, oxidases, copper, genetic transcription, Phanerochaete chrysosporium, white rot
    Source:
    Applied and environmental microbiology. Vol. 72, no. 7 (July 2006): p. 4871-4877.

  8. A novel extracellular multicopper oxidase from Phanerochaete chrysosporium with ferroxidase activity

    Lignin degradation by the white rot basidiomycete Phanerochaete chrysosporium involves various extracellular oxidative enzymes, including lignin peroxidase, manganese peroxidase, and a peroxide-generating enzyme, glyoxal oxidase. Recent studies have suggested that laccases also may be produced by this fungus, but these conclusions have been controversial. We identified...

    Author(s):
    Luis F. Larrondo; Loreto Salas; Francisco Melo; Rafael Vicuna; Daniel Cullen
    Year:
    2003
    Keywords:
    Phanerochaete chrysosporium, white rot, lignin degradation, extracellular multicopper oxidase, ferroxidase activity
    Source:
    Applied and environmental microbiology. Vol. 69, no. 10 (Oct. 2003): Pages 6257-6263

  9. Characterization of a multicopper oxidase gene cluster in Phanerochaete chrysosporium and evidence of altered splicing of the mco transcripts

    A cluster of multicopper oxidase genes (mco1, mco2, mco3, mco4) from the lignin-degrading basidiomycete Phanerochaete chrysosporium is described. The four genes share the same transcriptional orientation within a 25 kb region. mco1, mco2 and mco3 are tightly grouped, with intergenic regions of 2.3 and 0.8 kb, respectively, whereas mco4 is located 11 kb upstream of mco1...

    Author(s):
    Luis F. Larrondo; Bernardo Gonzalez; Dan Cullen; Rafael Vicuna
    Year:
    2004
    Keywords:
    Phanerochaete chrysosporium, gene transcripts, multicopper oxidase, gene splicing, gene clusters
    Source:
    Microbiology. Vol. 150 (2004): Pages 2775-2783

  10. Isolation and purification of pyranose 2-oxidase from Phanerochaete chrysosporium and characterization of gene structure and regulation

    Pyranose 2-oxidase (POX) was recovered from Phanerochaete chrysosporium BKM-F-1767 solid substrate culture using mild extraction conditions and was purified. 13C-nuclear magnetic resonance confirmed production of D- arabino -hexos-2-ulose (glucosone) from D-glucose with the oxidase. Peptide fingerprints generated by liquid chromatography-tandem mass spectrometry of...

    Author(s):
    Theodorus H. de Koker; Michael D. Mozuch; Daniel Cullen; Jill Gaskell; Philip J. Kersten
    Year:
    2004
    Keywords:
    Phanerochaete chrysosporium, pyranose 2-oxidase, gene structure, gene regulation, POX, isolation, purification
    Source:
    Applied and environmental microbiology. Vol. 70, no. 10 (Oct. 2004): Pages 5794-5800

  11. Structure and transcriptional impact of divergent repetitive elements inserted within Phanerochaete chrysosporium strain RP-78 genes

    We describe the structure, organization, and transcriptional impact of repetitive elements within the lignin-degrading basidiomycete, Phanerochaete chrysosporium. Searches of the P. chrysosporium genome revealed five copies of pce1, a 1,750-nt non-autonomous, class II element. Alleles encoding a putative glucosyltransferase and a cytochrome P450 harbor pce insertions...

    Author(s):
    Luis F. Larrondo; Paulo Canessa; Rafael Vicuna; Philip Stewart; Amber Vanden Wymelenberg; Dan Cullen
    Year:
    2007
    Keywords:
    Phanerochaete chrysosporium, retrotransposons, multicopper oxidase, cytochrome P450, fungi, genetics, industrial applications, molecular genetics, gene expression, copper, lignin, biodegradation, genetic transcription, Basidiomycetes, oxidases, wood-decaying fungi, transposons, white rot
    Source:
    Molecular genetics and genomics. Vol. 277 (2007): pages 43-55.

  12. A homokaryotic derivative of a Phanerochaete chrysosporium strain and its use in genomic analysis of repetitive elements

    Analysis of complex gene families in the lignin-degrading basidiomycete Phanerochaete chrysosporium has been hampered by the dikaryotic nuclear condition. To facilitate genetic investigations in P. chrysosporium strain BRM-F-1767, we isolated a homokaryon from regenerated protoplasts. The nuclear condition was established by PCR amplification of five unlinked genes...

    Author(s):
    Philip Stewart; Jill Gaskell; Daniel Cullen
    Year:
    2000
    Keywords:
    Phanerochaete chyrsosporium, Basidiomycotina, Genome analysis, Lignin, Strains, Wood destroying fungi, Decay fungi, Homokaryon
    Source:
    Applied and environmental microbiology. Vol. 66, no. 4 (Apr. 2000).:p. 1629-1633 : ill.

  13. Growth and fermentation responses of Phanerochaete chrysosporium to O2 limitation

    Phanerochaete chrysosporium BKM-F-1767 produced small amounts of ethanol from glucose, mannose, cellobiose, maltose and sucrose when grown with a limited O2 supply in sealed bottles. Under O 2 -limited growth on glucose, low levels of acetate or oxalate were produced when nitrogen was in excess or limited, respectively. Alcohol dehydrogenase activity (15 nmol/min/mg...

    Author(s):
    William R. Kenealy; Diane M. Dietrich
    Year:
    2004
    Keywords:
    Phanerochaete chrysosporium, fermentation, wood, ethanol, oxalate, white-rot fungi
    Source:
    Enzyme and microbial technology. Vol. 34 (2004): Pages 490-498.

  14. Genome sequence of the lignocellulose degrading fungus Phanerochaete chrysosporium strain RP78

    White rot fungi efficiently degrade lignin, a complex aromatic polymer in wood that is among the most abundant natural materials on earth. These fungi use extracellular oxidative enzymes that are also able to transform related aromatic compounds found in explosive contaminants, pesticides and toxic waste. We have sequenced the 30-million base-pair genome of...

    Author(s):
    Diego Martinez; Luis Larrondo; Nik Putnam; Maarten D. Sollewijn; Maarten D. Sollewijn Gelpke; Katherine Huang; Jarrod Chapman; Kevin G. Helfenbein; Preethi Ramaiya; J. Chris Detter; Frank Larimer; Pedro M. Coutinho; Bernard Henrissat; Randy Berka; Dan Cullen; Daniel Rokhsar
    Year:
    2004
    Keywords:
    Wood-destroying fungi, wood-decaying fungi, genome sequences, Phanerochaete chrysosporium
    Source:
    Nature biotechnology. Vol. 22, no. 6 (June 2004): Pages 695-700

  15. Differential expression in Phanerochaete chrysosporium of membrane- associated proteins relevant to lignin degradation

    Fungal lignin-degrading systems likely include membrane-associated proteins that participate in diverse processes such as uptake and oxidation of lignin fragments, production of ligninolytic secondary metabolites, and defense of the mycelium against ligninolytic oxidants. Little is known about the nature or regulation of these membrane-associated components. We grew...

    Author(s):
    Semarjit Shary; Alexander N. Kapich; Ellen A. Panisko; Jon K. Magnuson; Daniel Cullen; Kenneth E. Hammel
    Year:
    2008
    Keywords:
    Liquid chromatography, mass spectrometry, microbial metabolism, cellulose, membranes, proteins, fungi, Basidiomycetes, wood-decaying fungi, lignin, biodegradation, molecular genetics, gene expression, industrial applications, glucose, biotechnology, white rot, Phanerochaete chrysosporium
    Source:
    Applied and environmental microbiology. Vol. 74, no. 23 (Dec. 2008): pages 7252-7257.

  16. Transcriptome and secretome analyses of Phanerochaete chrysosporium reveal complex patterns of gene expression

    The wood decay basidiomycete Phanerochaete chrysosporium was grown under standard ligninolytic or cellulolytic conditions and subjected to whole-genome expression microarray analysis and liquid chromatography-tandem mass spectrometry of extracellular proteins. A total of 545 genes were flagged on the basis of significant changes in transcript accumulation and/or...

    Author(s):
    Amber J. Vanden Wymelenberg; Jill A. Gaskell; Michael D. Mozuch; Philip J. Kersten; Grzegorz Sabat; Diego Martinez; Daniel Cullen
    Year:
    2009
    Keywords:
    Liquid chromatography, cellulose, peptides, wood biodegradation, biodegradation, genomes, mass spectrometry, enzymes, biotechnology, lignocellulose, proteins, Basidiomycetes, molecular genetics, fungi, genetics, lignin, wood-decaying fungi, industrial applications, gene expression, manganese peroxidase, transcriptome, wood decay, secretome, Phanerochaete chrysosporium, white rot
    Source:
    Applied and environmental microbiology. Vol. 75, no. 12 (June 2009): pages 4058-4068.

  17. Lignin peroxidase gene family of Phanerochaete chrysosporium : complex regulation by carbon and nitrogen limitation and identification of a second dimorphic chromosome

    Lignin peroxidases (LiP) of Phanerochaete chrysosporium are encoded by a family of six closely related genes. Five LiP genes have been localized to the same dimorphic chromosome. In this investigation, relative transcript levels of the LiP genes were determined. Transcripts of the LiPA, LiPB, and 0282 genes were at similar levels in both carbon-and nitrogen-limited...

    Author(s):
    Philip Stewart; Philip Kersten; Amber J. Vanden Wymelenberg; Jill A. Gaskell; Daniel Cullen
    Year:
    1992
    Keywords:
    Biodegradation, enzymes, biotechnology, molecular genetics, fungi, genetics, lignin, Basidiomycetes, wood-decaying fungi, industrial applications, genetic transcription, Phanerochaete chrysosporium, peroxidases, gene expression, carbon, nitrogen, wood decay, white rot, dimorphic chromosomes, manganese peroxidase, glyoxal oxidase
    Source:
    Journal of bacteriology. Vol. 174, no. 15 (Aug. 1992): pages 5036-5042.

  18. Pyranose 2-oxidase from Phanerochaete chrysosporium : expression in E. coli and biochemical characterization

    The presented work reports the isolation and heterologous expression of the p2ox gene encoding the flavoprotein pyranose 2-oxidase (P2Ox) from the basidiomycete Phanerochaete chrysosporium. The p2ox cDNA was inserted into the bacterial expression vector pET21a(+) and successfully expressed in Escherichia coli. We obtained active, fully flavinylated recombinant P2Ox in...

    Author(s):
    Ines Pisanelli; Magdalena Kujawa; Oliver Spadiut; Roman Kittl; Petr Halada; Jindrich Volc; Michael D. Mozuch; Philip Kersten; Dietmar Haltrich; Clemens Peterbauer
    Year:
    2009
    Keywords:
    Pyranose 2-oxidase, Phanerochaete chrysosporium, lignocellulose degradation, heterologous expression, lignocellulose, biodegradation, enzymes, Basidiomycetes, molecular genetics, fungi, genetics, wood-decaying fung, industrial applications, gene expression, biotechnology, pyranose, Escherichia coli, Bacteria, flavoproteins, recombinant DNA, sugars, microbial metabolism, chemical kinetics, oxygen
    Source:
    Journal of biotechnology. Vol. 142, no. 2 (June 15, 2009): pages 97-106.

  19. Comparative transcriptome and secretome analysis of wood decay fungi Postia placenta and Phanerochaete chrysosporium

    Cellulose degradation by brown rot fungi, such as Postia placenta, is poorly understood relative to the phylogenetically related white rot basidiomycete, Phanerochaete chrysosporium. To elucidate the number, structure, and regulation of genes involved in lignocellulosic cell wall attack, secretome and transcriptome analyses were performed on both wood decay fungi...

    Author(s):
    Amber J. Vanden Wymelenberg; Jill Gaskell; Michael Mozuch; Grzegorz Sabat; John Ralph; Oleksandr Skyba; Shawn D Mansfield; Robert A. Blanchette; Diego Martinez; Igor Grigoriev; Philip J Kersten; Daniel Cullen
    Year:
    2010
    Keywords:
    Liquid chromatography, cellulose, biodegradation, mass spectrometry, enzymes, biotechnology, lignocellulose, Basidiomycetes, molecular genetics, fungi genetics, fungi, lignin, biodegradation, wood-decaying fungi, industrial applications, gene expression, genetic transcription, Postia placenta, Phanerochaete chrysosporium, brown rot, aspen, glucose, iron, quinone, chemical reactions, Populus, transcriptome, wood decay, secretome, white rot, decay fungi, Fenton system
    Source:
    Applied and environmental microbiology. Vol. 76, no. 11 (June 2010): p. 3599-3610.

  20. Influence of Populus Genotype on Gene Expression by the Wood Decay Fungus Phanerochaete chrysosporium

    We examined gene expression patterns in the lignin-degrading fungus Phanerochaete chrysosporium when it colonizes hybrid poplar (Populus alba �� tremula) and syringyl (S)-rich transgenic derivatives. Acombination ofmicroarrays and liquid chromatography- tandem mass spectrometry (LC-MS/MS) allowed detection of a total of 9,959 transcripts and 793...

    Author(s):
    Jill Gaskell; Amber Marty; Michael Mozuch; Philip J. Kersten; Sandra Splinter Bondurant; Grzegorz Sabat; Ali Azarpira; John Ralph; Oleksandr Skyba; Shawn D. Mansfield; Robert A. Blanchette; Dan Cullen
    Year:
    2014
    Keywords:
    Phanerochaete, lignin, Populus, transgenic, transcriptome, secretome
    Source:
    Appl. Environ. Microbiol. 2014, Volume 80, Number 18, 2014; pp. 5828-5835.

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