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  1. Comparison of multiple gene assembly methods for metabolic engineering

    A universal, rapid DNA assembly method for efficient multigene plasmid construction is important for biological research and for optimizing gene expression in industrial microbes. Three different approaches to achieve this goal were evaluated. These included creating long complementary extensions using a uracil-DNA glycosylase technique, overlap extension polymerase...

    Author(s):
    Chenfeng Lu; Karen Mansoorabadi; Thomas Jeffries
    Year:
    2007
    Keywords:
    Cloning, alcohol, fermentation, yeast, genetics, microbial metabolism, fungi, industrial applications, biotechnology, genetic engineering, yeast fungi, gene expression, multigene plasmid construction, gene assembly, xylose, decay fungi, Saccharomyces cerevisiae, ligation, optimization
    Source:
    Applied biochemistry and biotechnology. Vol. 136-140 (2007): pages 703-710.

  2. Molecular characterization of the Hansenula polymorpha FLD1 gene encoding formaldehyde dehydrogenase.

    Glutathione-dependent formaldehyde dehydrogenase (FLD) is a key enzyme required for the catabolism of methanol as a carbon source and certain primary amines, such as methylamine as nitrogen sources in methylotrophic yeasts. Here we describe the molecular characterization of the FLD1 gene from the yeast Hansenula polymorpha. Unlike the recently described Pichia pastoris...

    Author(s):
    Richard J. Baerends; Grietje J. Sulter; Thomas W. Jeffries; James M. Cregg; Marten Veenhuis
    Year:
    2002
    Keywords:
    Heterologous gene expression, methylotrophic yeast, methanol metabolism.
    Source:
    Yeast. Vol. 19 (2002): Pages 37-42.
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