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  1. Characterization of nonderivatized plant cell walls using high-resolution solution-state NMR spectroscopy

    A recently described plant cell wall dissolution system has been modified to use perdeuterated solvents to allow direct in-NMR-tube dissolution and high-resolution solution-state NMR of the whole cell wall without derivatization. Finely ground cell wall material dissolves in a solvent system containing dimethylsulfoxide-d6 and 1-methylimidazole-d6 in a ratio of 4:1 (v/...

    Author(s):
    Daniel J. Yelle; John Ralph; Charles R. Frihart
    Year:
    2008
    Keywords:
    NMR, HSQC, 1 methylimidazole-d6, dimethylsulfoxide-d6, Pinus taeda, Populus tremuloides, Hibiscus cannabinus, whole cell wall dissolution, lignin, polysaccharides, chemical reactions, polysaccharides, aspen, ultrastructure, kenaf, loblolly pine, plant cell walls, wood chemistry, cellulose, nuclear magnetic resonance spectroscopy, acetates, hemicellulose
    Source:
    Magnetic resonance in chemistry. Vol. 46 (2008): pages 508-517.

  2. Role of fungal peroxidases in biological ligninolysis

    The degradation of lignin by filamentous fungi is a major route for the recycling of photosynthetically fixed carbon, and the oxidative mechanisms employed have potential biotechnological applications. The lignin peroxidases (LiPs), manganese peroxidases (MnPs), and closely related enzymes of white rot basidiomycetes are likely contributors to fungal ligninolysis. Many...

    Author(s):
    Kenneth E. Hammel; Dan Cullen
    Year:
    2008
    Keywords:
    Genomes, fungi, genetics, oxidases, peroxidases, lignin, biodegradation, enzymes, biodegradation, industrial applications, fungal enzymes, biotechnology, Basidiomycetes, white rot
    Source:
    Current opinion in plant biology. Vol. 11 (2008): Pages 349-355

  3. Genome sequencing and analysis of the biomass-degrading fungus Trichoderma reesei (syn. Hypocrea jecorina)

    Trichoderma reesei is the main industrial source of cellulases and hemicellulases used to depolymerize biomass to simple sugars that are converted to...

    Author(s):
    Diego Martinez; Randy M. Berka; Bernard Henrissat; Markku Saloheimo; Mikko Arvas; Scott E. Baker; Jarod Chapman; Olga Chertkov; Pedro M. Coutinho; Daniel Cullen
    Year:
    2008
    Keywords:
    Cellulase, microbial metabolism, regulation, enzymes, industrial applications, biotechnology, gene expression, chemical engineering, alcohol, biodegradation, Trichoderma reesii, genetic engineering, nucleotide sequence, genomes, fungi, polysaccharides, biosynthesis, molecular genetics, Hypocrea jecorina, decay fungi, hemicellulose
    Source:
    Nature biotechnology. Vol. 26, no. 5: pages 553-560.
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