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  1. Two-Dimensional NMR Evidence for Cleavage of Lignin and Xylan Substituents in Wheat Straw Through Hydrothermal Pretreatment and Enzymatic Hydrolysis

    Solution-state two-dimensional (2D) nuclear magnetic resonance (NMR) spectroscopy of plant cell walls is a powerful tool for characterizing changes in cell wall chemistry during the hydrothermal pretreatment process of wheat straw for second-generation bioethanol production. One-bond 13C-1H NMR correlation spectroscopy, via...

    Author(s):
    Daniel J. Yelle; Prasad Kaparaju; Christopher G. Hunt; Kolby Hirth; Hoon Kim; John Ralph; Claus Felby
    Year:
    2012
    Keywords:
    wheat straw, hydrothermal, lignin, polysaccharides, O-acetyls, β-aryl ethers, Uronic acids, Cinnamates
    Source:
    Bioenerg. Res

  2. Characterizing lignin in a fungus-cultivating termite

    Lignin is a heterogenous plant cell wall polyphenol derived primarily from hydroxycinnamyl alcohols via combinatorial radical coupling reactions containing ether- and C-C linkages [2]. Its depolymerization is challenging, making lignin a major barrier to gaining access to stored energy within lignocellulosic materials [3]. Termites from the sub family ...

    Author(s):
    Hongjie Li; Daniel J. Yelle; John Ralph; Cameron R. Currie; Jianchu Mo
    Year:
    2018
    Keywords:
    Lignin, symbiosis, age polyethism, carbohydrate, NMR
    Source:
    In: Proceedings, polyphenols communication, the XXIXth international conference on polyphenols. 2 p.

  3. Fungal lignin peroxidase does not produce the veratryl alcohol cation radical as a diffusible ligninolytic oxidant

    Peroxidases are considered essential agents of lignin degradation by white-rot basidiomycetes. However, low-molecularweight oxidants likely have a primary role in lignin breakdown because many of these fungi delignify wood before its porosity has sufficiently increased for enzymes to infiltrate. It has been proposed that lignin peroxidases (LPs, EC 1.11.1.14) fulfill...

    Author(s):
    Carl J. Houtman; Eranda Maligaspe; Christopher G. Hunt; Elena Fernández-Fueyo; Angel T. Martínez; Kenneth E. Hammel
    Year:
    2018
    Keywords:
    White rot fungus, lignin biodegradation, peroxidase, aryl cation radical
    Source:
    Journal of Biological Chemistry. 293(13): 4702-4712.
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