Data on the effects of environmental pollution on carbon and nitrogen metabolism of one-, two-, and three-year-old needles and sapwood plugs collected in 2004 from Norway spruce from six forested sites within the Czech Republic

Metadata:

Entity_and_Attribute_Information:

Overview_Description:

Entity_and_Attribute_Overview:

Below you will find a list and description of the files included in this data publication.

VARIABLE DESCRIPTION FILE (1)

1. \Data\_variable_descriptions.csv: Comma-separated values (CSV) file containing a list and description of variables found in Czech_NS_data.csv. (A description of these variables is also provided in the metadata below.)

Columns include:

Filename = name of data file
Variable = name of variable
Units = units (if applicable)
Precision = precision (if applicable)
Description = description of variable
Notes = important notes regarding measurements
References = references for analytical methods


DATA FILES (1)

Note: -888.88 denotes data not available (na) and -999.99 denotes no analysis for that sample.

1. \Data\Czech_NS_data.csv: CSV containing geographic information and metabolic data (Free polyamines, free amino acids, exchangeable elements, chlorophyll, and soluble proteins) from foliage and sapwood plugs collected from six forested sites representing a pollution gradient in northern Czech Republic, 2004.

Variables include:

Location = Location of collection sites as designated by area, country (All six sites were located in the Czech Republic; four in the Krušné Hory (Ore) Mountains [Černý Potok, Kovářská, Načetín, Přebuz], and two in the Slavkov Forest [Lysina, Pluhův bor])

CollDate = Day & Year samples were collected (September 21-24, 2004)

Sitename = Sites within the Czech Republic where samples were collected (Aitkenhead-Peterson et al. 2006)
Černý Potok [site identifier: 50.4692, 13.0650]
Kovářská [site identifier: 50.4981, 13.1222]
Lysina [site identifier: 50.0353, 12.6706]
Načetín [site identifier: 50.5892, 13.2544]
Pluhův bor [site identifier: 50.0631, 12.7894]
Přebuz [site identifier: 50.3639, 12.5800]

Secsite = Secondary site designation where applicable
Site 35 at Černý Potok
Site 39 at Kovářská
Site 21 at Přebuz

Bedrock = Type of bedrock at each site as found in various publications (Krám et al. 2012; Hruška and Krám 2003; Oulehle et al. 2006; Aitkenhead-Peterson et al. 2006 and personal communications)
Gneiss
Granite
Leucogranite
Paragneiss
Serpentinite

Soil_type = Predominant soil type at each site as found in Aitkenhead-Peterson et al. (2006)
Distric cambisol
Entic spodozol
Entic spodozol or distric cambisol
Inceptisol
Spodo-distric cambisol

Lime = Whether site historically received dolomitic liming treatment
limed
no lime

Aspect = Aspect/exposure of each site
north-east
north-west
south-east
southerly

Altitude = Elevation of each site in meters (740-925 m)

Species = Species of tree that tissue was collected from (Norway spruce [Picea abies (L.) Karst])

Year_tissue = Needle age class_tissue type of collected samples
1Y_foliage = Current year foliage
2Y_foliage = Past season foliage
3Y_foliage = Third year foliage
sapwood = outer ~3 x 10 millimeters plug of sapwood with bark & cambium removed

Tree_no = Assigned tree #

Put = 5% Perchloric Acid (PCA) soluble Putrescine by High-Performance Liquid Chromatograph, fluorescence (HPLC), nmol g⁻¹ FW (nanoMoles Per Gram Fresh Weight) (Free Polyamines and amino acids were extracted and analyzed using methods found in Minocha and Long (2004) following guidance from EPA Compendium SW-846, Test Method 8000D.)

Spd = 5% PCA soluble Spermidine by HPLC, nmol g⁻¹ FW

Spm = 5% PCA soluble Spermine by HPLC, nmol g⁻¹ FW

SpdPut = Ratio of Spermidine to Putrescine, nmol g⁻¹ FW

Asp = 5% PCA soluble Aspartic Acid by HPLC, nmol g⁻¹ FW (*not available (na) in due to inconsistent contamination from ASN termination in dansylation process)

Glu = 5% PCA soluble Glutamic Acid by HPLC, nmol g⁻¹ FW

Gln = 5% PCA soluble Glutamine by HPLC, nmol g⁻¹ FW

Ser = 5% PCA soluble Serine by HPLC, nmol g⁻¹ FW

Arg_Thr = 5% PCA soluble Arginine+Threonine by HPLC, nmol g⁻¹ FW (*system was unable to separate these 2 amino acids)

Gly = 5% PCA soluble Glycine by HPLC, nmol g⁻¹ FW

Ala = 5% PCA soluble Alanine by HPLC, nmol g⁻¹ FW

Pro = 5% PCA soluble Proline by HPLC, nmol g⁻¹ FW

GABA = 5% PCA soluble γ-Aminobutyric Acid by HPLC, nmol g⁻¹ FW

Val = 5% PCA soluble Valine by HPLC, nmol g⁻¹ FW

Met = 5% PCA soluble Methionine by HPLC, nmol g⁻¹ FW

Ile = 5% PCA soluble Isoleucine by HPLC, nmol g⁻¹ FW

Leu = 5% PCA soluble Leucine by HPLC, nmol g⁻¹ FW

Ile_Leu = 5% PCA soluble Isoleucine+Leucine by HPLC, nmol g⁻¹ FW (*system was unable to separate these 2 amino acids)

Trp = 5% PCA soluble Tryptophan by HPLC, nmol g⁻¹ FW

Phe = 5% PCA soluble Phenylalanine by HPLC, nmol g⁻¹ FW

Cys = 5% PCA soluble Cysteine by HPLC, nmol g⁻¹ FW

Orn = 5% PCA soluble Ornithine by HPLC, nmol g⁻¹ FW

Lys = 5% PCA soluble Lysine by HPLC, nmol g⁻¹ FW

His = 5% PCA soluble Histidine by HPLC, nmol g⁻¹ FW

Ca = 5% Perchloric Acid (PCA) soluble Calcium by Inductively Coupled Plasma Optical Emission Spectrophotometer (ICP-OES), µmol g⁻¹ FW (microMoles Per Gram Fresh Weight) (5% Perchloric Acid soluble/exchangeable Ions were extracted and analyzed using ICP according to Minocha et al. (2010) following quidance in EPA compendium SW-846 Test Method 6010D.)

K = 5% PCA soluble Potassium by ICP-OES, µmol g⁻¹ FW

Mg = 5% PCA soluble Magnesium by ICP-OES, µmol g⁻¹ FW

Mn = 5% PCA solubleManganese by ICP-OES, µmol g⁻¹ FW

P = 5% PCA soluble Phosphorous by ICP-OES, µmol g⁻¹ FW

Al = 5% PCA soluble Aluminum by ICP-OES, µmol g⁻¹ FW

Fe = 5% PCA soluble Iron by ICP-OES, µmol g⁻¹ FW

Zn = 5% PCA soluble Zinc by ICP-OES, µmol g⁻¹ FW

TotChl = Total Chlorophyll by Ultraviolet/Visible Spectrophotometer, absorption, µg g⁻¹ FW (microGrams Per Gram Fresh Weight) (Chlorophyll was extracted in 95% ethanol (Minocha et al. 2009) and quantified according to Lichtenthaler (1987))

Chla = Chlorophyll A by spectrophotometer, µg g⁻¹ FW

Chlb = Chlorophyll B by spectrophotometer, µg g⁻¹ FW

Chlab = Ratio of Chlorophyll A/B

SolProt = Soluble proteins by Ultraviolet/Visible Spectrophotometer, absorption, mg g⁻¹ FW (milliGrams Per Gram Fresh Weight) (Soluble proteins were extracted in Tris buffer as found in Jones et al. (1989) and quantified using BioRad Protein Assay; based on Bradford Method (Bradford 1976))

Entity_and_Attribute_Detail_Citation:

Aitkenhead-Peterson, Jacqueline A.; Alexander, Jess E.; Albrechtová, Jana; Krám, Pavel; Rock, Barrett; Cudlín, Pavel; Hruška, Jakub; Lhotaková, Zuzana; Huntley, Ryan; Oulehle, Filip; Polák, Tomáš; McDowell, William H. 2006. Linking foliar chemistry to forest floor solid and solution phase organic C and N in Picea abies [L.] Karst stands in northern Bohemia. Plant and Soil, 283: 187–201. https://doi.org/10.1007/s11104-006-0010-7

Bradford, Marion M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein–dye binding. Analytical Biochemistry 72(1-2): 248-254. https://doi.org/10.1016/0003-2697(76)90527-3

Krám, Pavel; Hruška, Jakub; Shanley, James B. 2012. Streamwater chemistry in three contrasting monolithologic Czech catchments. Applied Geochemistry, 27(9): 1854-1863. https://doi.org/10.1016/j.apgeochem.2012.02.020

Jones, Clive G.; Hare, J. Daniel; Compton, Steven J. 1989. Measuring plant protein with the Bradford assay. Journal of Chemical Ecology 15: 979-992. https://doi.org/10.1007/BF01015193

Hruška, Jakub; Krám, Pavel. 2003. Modelling long-term changes in stream water and soil chemistry in catchments with contrasting vulnerability to acidification (Lysina and Pluhuv Bor, Czech Republic). Hydrology and Earth System Sciences, 7: 525-539. https://doi.org/10.5194/hess-7-525-2003

Lichtenthaler, Hartmut K. 1987. Chlorophylls and carotenoids: Pigments of photosynthetic biomembranes. Methods in Enzymology 148: 350-382. https://doi.org/10.1016/0076-6879(87)48036-1

Minocha, Rakesh; Long, Stephanie. 2004. Simultaneous separation and quantitation of amino acids and polyamines of forest tree tissues and cell cultures within a single high-performance liquid chromatography run using dansyl derivatization. Journal of Chromatography A. 1035: 63-73. https://doi.org/10.1016/j.chroma.2004.02.026

Minocha, Rakesh; Martinez, Gabriela; Lyons, Benjamin; Long, Stephanie. 2009. Development of a standardized methodology for quantifying total chlorophyll and carotenoids from foliage of hardwood and conifer tree species. Canadian Journal of Forest Research 39: 849-861. https://doi.org/10.1139/x09-015

Minocha, Rakesh; Long, Stephanie; Thangavel, Palaniswamy; Minocha, Subhash C.; Eagar, Christopher; Driscoll, Charles T. 2010. Elevation dependent sensitivity of northern hardwoods to Ca addition at Hubbard Brook Experimental Forest, NH USA. Forest Ecology and Management. 260: 2115-2125. https://doi.org/10.1016/j.foreco.2010.09.002

Oulehle, Filip; Hofmeister, Jeňýk; Cudlín, Pavel; Hruška, Jakub. 2006. The effect of reduced atmospheric deposition on soil and soil solution chemistry at a site subjected to long-term acidification, Načetín, Czech Republic. Science of The Total Environment, 370: 532-544. https://doi.org/10.1016/j.scitotenv.2006.07.031

Metadata_Reference_Information:

Metadata_Date: 20220621

Metadata_Contact:

Contact_Information:

Contact_Organization_Primary:

Contact_Organization: USDA Forest Service, Northern Research Station

Contact_Person: Rakesh Minocha

Contact_Position: Plant Physiologist

Contact_Address:

Address_Type: mailing and physical

Address: 271 Mast Road

City: Durham

State_or_Province: NH

Postal_Code: 03824

Country: USA

Contact_Voice_Telephone: 603-868-7622

Contact_Electronic_Mail_Address: rakesh.minocha@usda.gov

Contact_Information:

Contact_Organization_Primary:

Contact_Organization: USDA Forest Service, Northern Research Station

Contact_Person: Stephanie Long

Contact_Position: Biological Technician

Contact_Address:

Address_Type: mailing and physical

Address: 271 Mast Road

City: Durham

State_or_Province: NH

Postal_Code: 03824

Country: USA

Contact_Voice_Telephone: 603-868-7623

Contact_Electronic_Mail_Address: stephanie.long2@usda.gov

Metadata_Standard_Name: FGDC Biological Data Profile of the Content Standard for Digital Geospatial Metadata

Metadata_Standard_Version: FGDC-STD-001.1-1999