Title:

Fluorescence emission images of wood stained with acridine orange

Author(s):

Houtman, Carl J.; Hunt, Chris G.; Hammel, Ken E.

Publication Year:

2016

How to Cite:

These data were collected using funding from the U.S. Government and can be used without additional permissions or fees. If you use these data in a publication, presentation, or other research product please use the citation below when citing the data publication:

Houtman, Carl J.; Hunt, Chris G.; Hammel, Ken E. 2016. Fluorescence emission images of wood stained with acridine orange. Fort Collins, CO: Forest Service Research Data Archive. https://doi.org/10.2737/RDS-2016-0022

Abstract:

Colonization of wood blocks, Picea glauca, by brown and white rot fungi, Postia placenta, Phanerochaete chrysosporium, and Serpula lacrymans, rapidly resulted in detectable wood oxidation, as shown by a reduced phloroglucinol response, a loss of autofluorescence, and acridine orange (AO) staining. The data included in this archive were used to refine a method for detecting changes in the chemical properties of wood. This method involves staining the sample with acridine orange and then observing the light emission using a fluorescence microscope. Data include fluorescence emission microscopic images of AO-stained wood, transmission microscopic images of phloroglucinol-stained wood, isothermal titration calorimetry results, and fluorescence emission spectra.

Keywords:

biota; Forest & Plant Health; Mycology; Forest Products; Wood chemistry; fluorescence microscopy; acridine orange; oxidation; wood; fungus; Madison; Wisconsin

Related publications:

• Houtman, Carl J.; Kitin, Peter; Houtman, Jon C. D.; Hammel, Kenneth E.; Hunt, Chrisopher G. 2016. Acridine orange indicates early oxidation of wood cell walls by fungi. PLoS ONE. 11(7):e0159715. https://doi.org/10.1371/journal.pone.0159715

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