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Publication Details

Title:
Nitrogen fixation by Robinia pseudoacacia (L.) seedlings in the greenhouse under manipulated nitrogen and light treatments
Author(s):
Ottinger, Sarah L.; Miniat, Chelcy Ford; Wurzburger, Nina
Publication Year:
2023
How to Cite:
These data were collected using funding from the U.S. Government and can be used without additional permissions or fees. If you use these data in a publication, presentation, or other research product please use the following citation:
Ottinger, Sarah L.; Miniat, Chelcy Ford; Wurzburger, Nina. 2023. Nitrogen fixation by Robinia pseudoacacia (L.) seedlings in the greenhouse under manipulated nitrogen and light treatments. Fort Collins, CO: Forest Service Research Data Archive. https://doi.org/10.2737/RDS-2023-0007
Abstract:
Symbiotic nitrogen fixation (SNF) is a critical mechanism of ecosystem recovery, and in forests of the eastern United States, the most common tree species that supports SNF is black locust (Robinia pseudoacacia L.). Despite its prevalence, black locust’s fixation strategy—whether it maintains fixation at a constant rate (obligate fixation) or reduces its fixation rate (facultative fixation)—is unknown. In this study we examined how nitrogen (N) and light control SNF by black locust, by growing seedlings under two nitrogen levels (nitrogen added and nitrogen not added) and across four levels of light (10%, 20%, 40%, and 100%), with 12-13 replicates for each nitrogen x light treatment. This experiment, conducted at the Coweeta Hydrologic Laboratory in North Carolina, started in May 2020 and seedlings were harvested at the end of 12 weeks. This data publication includes the biomass measurements, net photosynthesis, and nodule activity for each replicate from each of 8 light transmittance and nitrogen treatments.

Keywords:
biota; Ecology, Ecosystems, & Environment; Plant ecology; Soil; Forest & Plant Health; Robinia pseudoacacia (black locust); succession; disturbance; nodule; rhizobia; ecology; vegetation; early successional; nitrogen fixation; nodules; aboverground biomass; belowground biomass; net photosynthesis; acetylene reduction assay; 15N2 incubations; Coweeta Hydrologic Laboratory; North Carolina; Southern Appalachians; Appalachian Mountains
Related publications:
  • Ottinger, Sarah L.; Miniat, Chelcy F.; Wurzburger, Nina. 2023. Nitrogen and light regulate symbiotic nitrogen fixation by a temperate forest tree. Oecologia. 2023: 1-10. https://doi.org/10.1007/s00442-023-05313-0
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